Table 1.
Primer sequence of tumor associated macrophage (TAM) marker.
Table 2.
Clinical information of 148 WT patients.
Fig 1.
Recruitment of TAM in WT and paracancerous tissue.
(A-C) mRNA expression of M2 TAM markers; (D) mRNA expression of total macrophage marker; (E-F) mRNA expression of M1 TAM markers (*p<0.05; **p<0.01; p<0.05 was considered statistically significant).
Fig 2.
IF detection of TAM recruitment in Wilms tumor tissue.
(A) Representative immunofluorescence images of CD68, CD163, and CD86 staining in WT tissue sections; (B) Recruitment of CD68+CD163+M2 macrophages in Wilms tumor of non-chemotherapy group; (C) Recruitment of CD68+CD86+M1 macrophages in Wilms tumor of non-chemotherapy group; (D) Recruitment of CD68+CD163+M2 macrophages in Wilms tumor of chemotherapy group; (E) Recruitment of CD68+CD86+M1 macrophages in Wilms tumor of chemotherapy group (Red: overall macrophage Mtotal; Green: Figure B and D are M2 macrophages, Figure C and E are M1 macrophages) (50 μm Ruler).
Fig 3.
The recruitment difference of TAM in different stages WT tissue.
(A-C) The recruitment difference of TAM in different stages WT in the non-chemotherapy group; (D) The recruitment difference of overall macrophage Mtotal in WT tissue of the chemotherapy group; (E-G) The recruitment difference of TAM in WT tissues between non-chemotherapy and chemotherapy group during the same stage (*p<0.05; **p<0.01; p<0.05 was considered statistically significant).
Table 3.
The recruitment difference of TAM in WT of non-chemotherapy group.
Table 4.
The recruitment difference of overall macrophage Mtotal in WT tissues of the chemotherapy group.
Table 5.
The recruitment difference of M1 and M2 in WT tissue of the chemotherapy group.
Fig 4.
(A-C) The relationship between TAM recruitment and prognosis of WT patients in non-chemotherapy group; (D-F) The relationship between TAM recruitment and prognosis of WT patients in chemotherapy group (p<0.05 was considered statistically significant).
Table 6.
COX multivariate regression analyzed prognostic factors in WT patients of non-chemotherapy group.
Table 7.
COX multivariate regression analyzed prognostic factors in WT patients of chemotherapy group.