Fig 1.
Strategy for QTL analysis in Pleurotus ostreatus.
Linkage grouping was accomplished through polymorphisms of SSRs in a segregated haploid population (HMmp). Phenotyping was performed in the dikaryotic population (SGHMmp) obtained by mating a compatible tester with a segregated haploid population. QTL analysis was performed using linkage information and traits data.
Table 1.
Phenotypic characteristics and broad-sense heritability of the segregating population crossed with the tester.
Fig 2.
Location of QTLs responsible for traits on the Pleurotus ostreatus linkage map with LOD score.
The genetic distance of markers in cM (Kosambi units) is indicated on the left side. The QTL nomenclature is described in the materials and methods section. The confidence interval is denoted by a bar (> LOD-1) and a line (> LOD-2) on the genetic map.
Table 2.
QTLs for yield and morphological traits found in the fruiting body of SGHMmp.
Fig 3.
Transcript levels of candidate genes in high cap yellowness and low cap yellowness strains of Pleurotus ostreatus.
Each set of three hybrids with high and low cap yellowness was selected from a second segregated population (HMmp2). Relative gene expression was estimated by the comparative 2−ΔΔCt method and was relative to the control using gene-specific primers. Gene expression was normalized to beta-tubulin expression and calibrated to the value for the high cap yellowness set, which was assigned a value of 1 (for A; B setting is the opposite), using the standard curve method (ABI). All assays were performed in triplicate. The error bars show the standard deviations for triplicate samples. The asterisk on the histogram indicates that the result is considered statistically significant (*, P < 0.05; **, P < 0.01; ***, P < 0.001). A, JHH00315-7 and JHH00323: Glutathione- S-transferase; B, JHH00313: glutathione disulfide reductase, HMS00363: Hydroxyacylglutathione hydrolase, HMS09261: Cystathionine beta-synthase, and JHH00337: MYB transcription factor.