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Fig 1.

Neighbour-joining tree for the sublineages of Liodessus quillacinga.

Green L. quillacinga ecuadoriensis ssp. nov., Blue L. quillacinga cochaensis, Pink L. quillacinga quillacinga, Red L. quillacinga cumbalis, Black outgroup.

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Fig 1 Expand

Fig 2.

Maps of A sampling localities and B lineages of Liodessus quillacinga. Blue coloured areas represent Páramo habitats.

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Fig 2 Expand

Fig 3.

Habitus of A L. quillacinga quillacinga holotype (HT) and paratype (PT), B L. quillacinga cochaensis holotype (HT) and paratype (PT), C L. cumbalis holotype (HT) and paratype (PT), D–F L. quillacinga ecuadoriensis ssp. nov. paratypes from different localities. Scale bar: 1 mm.

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Fig 3 Expand

Fig 4.

Median lobes in lateral view of Liodessus species A L. quillacinga quillacinga holotype (HT) and paratype (PT), B L. quillacinga cochaensis holotype (HT) and paratype (PT), C L. cumbalis holotype (HT) and D–G L. quillacinga ecuadoriensis ssp. nov. paratypes from different localities. Scale bar: 0.3 mm.

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Fig 4 Expand

Fig 5.

Median lobes in ventral view of Liodessus species A L. quillacinga cumbalis holotype (HT), B L. quillacinga quillacinga holotype (HT) and paratype (PT), B L. quillacinga cochaensis holotype (HT) and paratype (PT), D–H L. quillacinga ecuadoriensis ssp. nov. paratypes from different localities. Scale bar: 0.1 mm.

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Fig 5 Expand

Fig 6.

Haplotype network for Liodessus quillacinga, including information of main sublineages.

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Fig 6 Expand

Fig 7.

Heatmap of pairwise comparison of Fst values among L. quillacinga sublineages.

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Fig 7 Expand

Table 1.

Nucleotide diagnostic characters in the COI alignment for sublineages of Liodessus quillacinga (above) and Liodessus quimbaya (below).

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Table 1 Expand

Fig 8.

Holotype of Liodessus quillacinga ecuadoriensis ssp. nov. from the Laguna San Pablo, 2,700 m, 0.199° -78.2331°.

Scale bar: 1 mm.

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Fig 8 Expand

Fig 9.

A–D Habitus of Liodessus quillacinga ecuadoriensis ssp. nov. paratypes from different localities.

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Fig 10.

Habitats of Liodessus quillacinga ecuadoriensis ssp. nov., A Otavalo, Laguna San Pablo (locus typicus); B Mt. Cotopaxi, small wet patch; C Mt. Antisana, La Mica area.

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Fig 10 Expand

Fig 11.

Neighbour-joining tree for the sublineages of Liodessus quimbaya.

Blue L. quimbaya azufralis stat. nov., Green L. quimbaya quimbaya, Pink L. quimbaya paletara ssp. nov., Black outgroup.

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Fig 11 Expand

Fig 12.

Maps of A sampling localities and B lineages of Liodessus quimbaya. Blue coloured areas represent Páramo habitats.

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Fig 12 Expand

Fig 13.

Habitus of A L. quimbaya azufralis stat. nov. paratypes (PT), B L. quimbaya quimbaya holotype (HT), C–D L. quimbaya azufralis stat. nov. from different localities. Scale bar: 1 mm.

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Fig 14.

Habitus of A L. quimbaya quimbaya from Malvaza, B L. quimbaya paletara ssp. nov. paratypes and holotype (HT), C–D L. quimbaya azufralis stat. nov. from different localities. Scale bar: 1 mm.

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Fig 14 Expand

Fig 15.

Median lobes in lateral view of A L. quimbaya azufralis stat. nov. holotype (HT) and paratype (PT), B–D from Ecuador; E L. quimbaya quimbaya holotype (HT) and paratype (PT), F L. quimbaya paletara ssp. nov. Scale bar: 0.3 mm.

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Fig 15 Expand

Fig 16.

Median lobes in lateral view of Liodessus species A L. quimbaya paletara ssp. nov. paratype and holotype (HT), B L. quimbaya quimbaya from Malvaza. Scale bar: 0.3 mm.

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Fig 17.

Median lobes in ventral view of Liodessus species A L. quimbaya quimbaya holotype (HT) and paratype (PT), B L. quimbaya azufralis stat. nov. holotype (HT) and paratype (PT), C L. quimbaya paletara ssp. nov. holotype (HT) and paratype (PT), D L. quimbaya quimbaya from Malavaza, E–I L. quimbaya azufralis stat. nov. from different localities. Scale bar: 0.1 mm.

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Fig 17 Expand

Fig 18.

Haplotype network for Liodessus quimbaya, including information of main sublineages.

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Fig 18 Expand

Fig 19.

Heatmap of pairwise comparison of Fst values among L. quimbaya sublineages.

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Fig 19 Expand