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Fig 1.

3D-Interaction diagram of (a) GDP-fucose, (b) N-acetyllactosamine, indicating non-covalent interactions with conserved residues of FUT2 (black dotted lines: hydrogen bonds, cyan dotted lines: π-π stacking interactions).

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Fig 1 Expand

Fig 2.

3D-Docked poses of enalaprilat dehydrate (1) in the (a) donor, and (b) acceptor domains of FUT2 (Black dotted lines: hydrogen bonds, cyan dotted lines: π-π stacking interactions).

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Fig 2 Expand

Fig 3.

3D-Docked poses of ibuprofen (2) in the (a) donor, and (b) acceptor domains of FUT2 (Black dotted lines: hydrogen bonds, dark green dotted lines: π cationic interactions, light green: salt bridge).

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Fig 3 Expand

Fig 4.

3D-Docked poses of ascorbic acid (3) in the (a) donor, and (b) acceptor domains of FUT2 (Black dotted lines: hydrogen bonds, light green: salt bridge).

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Fig 4 Expand

Fig 5.

3D-Docked poses of acarbose (4) in the (a) donor, and (b) acceptor domains of FUT2 (Black dotted lines: hydrogen bonds, light green: salt bridge).

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Fig 5 Expand

Fig 6.

3D-Docked poses of ceftriaxone sodium (5) in the (a) donor, and (b) acceptor domains of FUT2 (Black dotted lines: hydrogen bonds, light green: salt bridge, red: aromatic hydrogen bonds).

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Fig 6 Expand

Fig 7.

(A) Reference 1H-NMR spectrum of enalaprilat dehydrate (1). (B) Difference spectrum (STD-NMR) of enalaprilat dihydrate with FUT2.

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Fig 7 Expand

Fig 8.

(A) Reference 1H-NMR spectrum of ibuprofen (2). (B) Difference spectrum (STD-NMR) of ibuprofen with FUT2. (*) = Solvent peak.

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Fig 8 Expand

Fig 9.

(A) Reference 1H-NMR spectrum of ascorbic acid (3). (B) Difference spectrum (STD-NMR) of ascorbic acid (3) with FUT2.

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Fig 9 Expand

Fig 10.

(A) Reference 1H-NMR spectrum of acarbose (4). (B) Difference spectrum (STD-NMR) of acarbose. with FUT2.

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Fig 10 Expand

Fig 11.

(A) Reference 1H-NMR spectrum of ceftriaxone sodium (5). (B) Difference spectrum (STD-NMR) of ceftriaxone sodium with FUT2.

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Fig 11 Expand

Fig 12.

The molecular dynamics trajectories of enalaprilat dihydrate with proteins acceptor (a, c, e, g) and donor binding sites (b, d, f, h). (a, b) Protein-ligand RMSD (c, d) Protein RMSF (e, f) Ligand RMSF, (g, h) Protein-ligand interaction histogram.

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Fig 12 Expand

Fig 13.

The molecular dynamics trajectories of ibuprofen (2) with protein acceptor (a, c, e, g) and donor binding sites (b, d, f, h). (a, b) Protein-ligand RMSD (c, d) Protein RMSF (e, f) Ligand RMSF, (g, h) Protein-ligand interactions histogram.

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Fig 13 Expand

Fig 14.

The molecular dynamics trajectories of ascorbic acid with protein acceptor (a, c, e, g) and donor binding sites (b, d, f, h). (a, b) Protein-ligand RMSD (c, d) Protein RMSF (e, f) Ligand RMSF (g, h) Protein-ligand interaction histogram.

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Fig 14 Expand

Fig 15.

The molecular dynamics trajectories of acarbose (4) with protein acceptor (a, c, e, g) and donor binding sites (b, d, f, h). (a, b) Protein-ligand RMSD (c, d) Protein RMSF (e, f) Ligand RMSF, (g, h) Protein-ligand interaction histogram.

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Fig 15 Expand

Fig 16.

The molecular dynamics trajectories of ceftriaxone sodium with protein acceptor (a, c, e, g) and donor binding sites (b, d, f, h). (a, b) Protein-ligand RMSD (c, d) Protein RMSF (e, f) Ligand RMSF, (g, h) Protein-ligand interaction histogram.

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Fig 16 Expand