Fig 1.
UniProt neighbor joining tree.
Neighbor-joining tree constructed using the constant heavy regions of the longest manatee IgA as a query at UniProt. The tree reveals a notable proximity between the orders Sirenia and Primates, with identities surpassing 69%.
Table 1.
Selected Foldseek results for six different species.
Fig 2.
Stuctural overlap between manatee and human IgA.
Structural overlaps obtained with Foldseek. (a.) Manatee IgA (Blue) vs. Human IgA1 (Yellow). (b.) Manatee IgA (Blue) vs. Human IgA2. CH2 and CH3 exhibit the highest structural similarity. CH1 is not as close as expected and this might be due to the interdomain region (arrow) which is longer in humans and might be affecting the overlapping. However, the CH1 itself is very similar.
Fig 3.
Linear and conformational epitope prediction.
Predicted linear (red) and conformational epitopes (blue) within the longest manatee IgA sequence. Panel a show a front view and panel b shows a rear view.
Table 2.
Conservation, identity and similarity of predicted epitopes along manatee IgAs.