Fig 1.
CT26- luc cell viability is affected by increasing amount of gel on D4 after seeding.
A photograph of the cell cultures on D4 according to gel percentages (A). The percentages of viable cells on D4. Mean +-SD. * for p value <0.05, ** <0.01, *** <0.001 and **** <0.0001. (B). Logarithmic regression curve between gel concentration and viability indicates an IC50 around 7% of HayRegen® gel (C).
Table 1.
Statistic tests for in vitro cell viability regarding gel concentration.
A one-way analysis of variance (ANOVA) with Tukey test for multiple comparison confirmed a significative effect on cell viability at high concentration of HyaRegen® gel (F (8, 45) = 11,21 P<0,0001).
Fig 2.
In vivo analysis of HyaRegen® Gel effects on tumor proliferation, dissemination and ascites.
In vivo quantification of tumor growth via bioluminescence tracking of luciferase-expressing CT26 cells on days 11, 17, and 21 after gel administration: BLI mice photography (A) and tumor growth histograms, mean +-SD,* for p value <0.05 (B). Comparison of ascites volume between the control and gel groups on D21, mean +-SD (C). Comparison of median peritoneal cancer index (PCI) between the control and gel groups (D).
Fig 3.
Histograms showing in vivo proliferating cells (Ki-67 antigen labeling) for the control and gel groups, mean +-SD (A). Micrography showing Ki-67 (B) and HES staining (C).