Fig 1.
In vitro regeneration in L. akkusianum.
(A) Explant preparation. (B) Adventitious bud regeneration in M0 medium 5–6 weeks of culture in the dark. (C) Callus and adventitious buds regeneration in M9 medium 5–6 weeks of culture. (D) Callus and adventitious buds regeneration in M10 medium 5–6 weeks of culture. (E) Shoots development and proliferation under 16/8 h photoperiod (F) Plantlets with well-developed roots. (G) Acclimatized plantlets in the grow box. (H) Plantlets 2 months after transfer into the peat-perlite substrate (1:1 v/v). (I) DNA bands profile of in vitro regenerated plantlets (2–7) and mother plant (1). Scale bars: B, C, D, E = 10 mm; A, F, G = 1 cm; H = 5 cm.
Table 1.
ISSR primer sequence [40].
Table 2.
Callus induction rate and adventitious bud number per explants cultured on the MS medium supplemented with various concentrations of NAA and BA or mT.
Fig 2.
Effects of plant growth regulator combination on shoot number, fresh shoot length and shoot weight in the sixth week of culture (*p < 0.05, LSD Shoot Number: 0.876; Levels not connected by the same letter are significantly different).
Table 3.
Effects of different NAA and IBA on rooting behavior and survival rate of plantlets.
Fig 3.
Actual against predicted values of (A) adventitious bud, (B) number of roots, (C) root length, (D) number of shoots, (E) shoot length, (F) shoot weight using MLP model analysis.
Fig 4.
Actual against predicted values of (A) adventitious bud, (B) number of roots, (C) root length, (D) number of shoots, (E) shoot length, (F) shoot weight using XGBoost model analysis.
Table 4.
Performance metrices of the MLP and XGBoost models.