Fig 1.
Surface ECG metrics for mice in SQ and SMP groups treated with ISP.
For all graphs, both SQ and SMP treated groups are presented in the same X axis but are separated by a dotted line. SQ group is on left side and SMP group is on right side of dotted line. Symbols represent number of mice in each cohort (n = 4). A) heart rate, B) RR interval, C) PR interval, D) P duration, E) QRS interval, F) QT interval, G) P amplitude, H) Q amplitude, I) R amplitude, and J) S amplitude. Each boxplot represents min to max showing all points, line at median and plus sign at mean. RR interval, heart rate, P duration, Q, R and S amplitude data were analyzed using ordinary one-way ANOVA with Sidak’s multiple comparison’s test. PR, QRS, and QT interval, and P amplitude data were analyzed using Kruskal-Wallis ANOVA with Dunn’s multiple comparison’s test. * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001.
Fig 2.
Heart function assessed via echocardiography.
For all graphs, symbols represent the number of mice in each cohort (n = 8). A) % ejection fraction, B) % fractional shortening, C) diastolic left ventricular anterior wall thickness, D) diastolic left ventricular posterior wall, E) diastolic left ventricular internal diameter, F) left ventricular end diastolic volume, G) left ventricular mass, saline treated mice only, F) left ventricular mass SQ only, and G) left ventricular mass SMP only. In A-F, both SQ and SMP treated groups are presented in the same X axis but are separated by a dotted line. SQ group is on left side of dotted line and SMP group is on right side. Each boxplot represents min to max showing all points, line at median and plus sign at mean. Data in A, C, E-G were analyzed using ordinary one-way ANOVA with Sidak’s multiple comparison’s test and data in B and D were analyzed using Kruskal-Wallis ANOVA with Dunn’s multiple comparison’s test. * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001.
Fig 3.
Body, lung, and heart weights.
Top of figure contains legend. For A-I, symbols represent number of mice in each cohort (n = 8). A-C show saline treated mice only. A) % body weight change over 2 weeks, B) lung weight, C) heart weight over tibia length. Each boxplot represents min to max showing all points, line at median and plus sign at mean. Data were analyzed with unpaired t-test. D-I show saline, 2, 4, or 10mg/kg ISP treated mice. On the left, SQ treated mice and on the right, SMP treated mice. D) SQ group % body weight change, E) SMP group % body weight change, F) SQ group lung weight, G) SMP group lung change, H) SQ group heart weight over tibia length, and I) SMP group heart weight over tibia length. Each boxplot represents min to max showing all points, line at median and plus sign at mean. Data were analyzed using ordinary one-way ANOVA with Sidak’s multiple comparison’s test. * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001.
Fig 4.
Gene expression changes after 2 weeks of ISP treatment via SQ and SMP.
For A-E, symbols represent number of mice in each cohort (n = 3–4). On the left of dotted line, triangles represent mice treated via SQ and on the right of dotted line, circles represent mice treated via SMP. A) Acta2, B) Myh7 C) Postn, D) Nppa, and E) Nppb. Each boxplot represents min to max showing all points, line at median and plus sign at mean. Data were analyzed using ordinary two-way ANOVA with Tukey’s multiple comparison’s test. * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001.