Fig 1.
Step-by- step workflow of applied pipeline in current study.
Fig 2.
The relationship of Soft Threshold (power) with Scale Free Topology (A) and Mean Connectivity (B).
The number showing the power threshold for each soft power corresponding to the SFT and connectivity mean.
Fig 3.
Weighted gene co-expression network analysis of stress responses genes in Chromochloris zofingiensis.
(A) Determined functional modules based on hierarchical cluster tree of the Meta genes. (B) The sizes of determined modules based on the number of involved genes. (C) The module Eigen gene adjacency estimated by hierarchical clustering and shown by heat map. (B) Topological Overlap Matrix (TOM) value among the proteins of the network delimited in modules by the dynamic method.
Fig 4.
Preservation analysis of co-expressed modules of control networks were tested in the stress condition based on the median rank (A) and Zsummary (B) algorithms.
Each dots indicated the corresponding modules which was defined based on WGCNA method.
Fig 5.
Functional impact of non-preserved modules-based gene ontology (biological processes category).
Table 1.
The significantly enriched KEGG pathways in non-preserved module under stress condition.
Fig 6.
Regulatory networks between Hubs and TFs of non-preserved co-expressed modules under stress condition.
Each dots indicated the genes involved in coexpressed modules. Here in we defined the dot as a node in the constructed network. Node colors represent correspondence module color of hub genes. TFs were shown with bigger red nodes.
Table 2.
Identified transcription factors (TFs) and corresponding non-preserved modules.