Fig 1.
Sequence for the prevention protocol.
Fig 2.
Sequence for the disinfection protocol.
Fig 3.
CFU/mL values of C. albicans and S mutans cells (single and dual-species biofilms) referring to biofilm formation on denture base (A and C) and reline acrylic resin (B and D) samples after immersion in PBS, Lifebuoy, and 0.5% sodium hypochlorite solutions.
Fig 4.
Results of the cellular metabolism of the C. albicans and S. mutans cells (single and dual-species biofilms) referring to biofilm formation on denture base (A-C) and reline acrylic resin (D-F) samples after immersion in PBS, Lifebuoy, and 0.5% sodium hypochlorite solutions.
Fig 5.
CFU/mL values of single (A and B) and dual-species (C and D) biofilms formed on the denture base and reline acrylic resin samples, submitted to the disinfection procedure.
Fig 6.
Results of the cellular metabolism of the C. albicans and S. mutans cells (single and dual-species biofilms) formed on denture base (A-C) and reline acrylic resins (D-F) samples.
Fig 7.
Cell viability of C. albicans and S. mutans single and dual-specie biofilms analyzed by CLSM on denture base acrylic resin samples.
Samples labeled with Syto-9 (cells marked in green) and propidium iodide (cells marked in red). (A, B) C. albicans biofilm–PBS (Negative control); (C, D) C. albicans biofilm–Lifebuoy solution; (E) C. albicans biofilm–Sodium Hypochlorite 0,5% (Positive control); (F, G) S. mutans biofilm—PBS (Negative control); (H, I) S. mutans biofilm—Lifebuoy solution; (J) S. mutans biofilm—Sodium Hypochlorite 0,5% (Positive control); (K, L) Dual-species biofilm—PBS (Negative control); (M, N) Dual-species biofilm—Lifebuoy solution; (O) Dual-species biofilm–Sodium Hypochlorite 0,5% (Positive control).
Fig 8.
Cell viability of C. albicans and S. mutans single and dual-specie biofilms analyzed by CLSM on hard reline acrylic resin samples.
Samples labeled with Syto-9 (cells marked in green) and propidium iodide (cells marked in red). (a, b) C. albicans biofilm–PBS (Negative control); (c, d) C. albicans biofilm–Lifebuoy solution; (e) C. albicans biofilm–Sodium Hypochlorite 0,5% (Positive control); (f, g) S. mutans biofilm—PBS (Negative control); (h, i) S. mutans biofilm—Lifebuoy solution; (j) S. mutans biofilm—Sodium Hypochlorite 0,5% (Positive control); (k, l) Dual-species biofilm—PBS (Negative control); (m, n) Dual-species biofilm—Lifebuoy solution; (o) Dual-species biofilm–Sodium Hypochlorite 0,5% (Positive control).
Fig 9.
Assessment of damage to the protein components of the biofilm extracellular matrix after treatment protocols on denture base acrylic resin samples.
Components visualized in red were marked with Sypro Ruby Biofilm Matrix Stain. (A) C. albicans biofilm–PBS (Negative control); (B) C. albicans biofilm–Lifebuoy solution; (C) C. albicans biofilm–Sodium Hypochlorite 0,5% (Positive control); (D) S. mutans biofilm—PBS (Negative control); (E) S. mutans biofilm—Lifebuoy solution; (F) S. mutans biofilm—Sodium Hypochlorite 0,5% (Positive control); (G) Dual-species biofilm—PBS (Negative control); (H) Dual-species biofilm—Lifebuoy solution; (I) Dual-species biofilm–Sodium Hypochlorite 0,5% (Positive control).
Fig 10.
Assessment of damage to the protein components of the biofilm extracellular matrix after treatment protocols on hard reline acrylic resin samples.
Components visualized in red were marked with Sypro Ruby Biofilm Matrix Stain. (a) C. albicans biofilm–PBS (Negative control); (b) C. albicans biofilm–Lifebuoy solution; (c) C. albicans biofilm–Sodium Hypochlorite 0,5% (Positive control); (d) S. mutans biofilm—PBS (Negative control); (e) S. mutans biofilm—Lifebuoy solution; (f) S. mutans biofilm—Sodium Hypochlorite 0,5% (Positive control); (g) Dual-species biofilm—PBS (Negative control); (h) Dual-species biofilm—Lifebuoy solution; (i) Dual-species biofilm–Sodium Hypochlorite 0,5% (Positive control).