Fig 1.
The red raspberry at different development stage [12].
Table 1.
Primer sequence information verified by qRT-PCR.
Table 2.
Fluorescence quantitation primer sequences.
Table 3.
Summary of sample sequencing output statistics.
Fig 2.
Correlation heat map; sample differences between groups and the sample repetition within groups.
The left and upper sides are sample clustering, the right and lower sides are sample names, and the squares of different colors represent the correlations of the two samples.
Fig 3.
Quantitative real-time polymerase chain reaction analysis of the expression of secondary metabolism-related genes (Analyses were completed in triplicate) 1: The green fruit from Polka; 2: The ripe fruit from Polka; 3: The green fruit from Orange legend; 4: The ripe fruit from Orange legend.
Table 4.
Success rate of genes annotated in seven databases.
Fig 4.
Comparison of NR protein database annotated genes with other species.
Fig 5.
Functional annotation and classification based on KOG database.
Fig 6.
Functional annotation and classification based on KEGG database.
Fig 7.
Differentially expressed genes of red raspberry in different comparison groups; BQ: The green fruit from Polka; BH: The ripe fruit from Polka; CQ: The green fruit from Orange legend; CH: The ripe fruit from Orange legend.
Table 5.
Analysis of known genes of phenylpropane metabolism pathway in red raspberry transcriptome.
Fig 8.
Over-expression of RinPKS4 gene in red raspberry.
A: RinPKS4 expression analysis of each tissue part of “Polka”; B: RinPKS4 expression analysis of each tissue part of “Orange legend”.
Fig 9.
Relative expression level of RinPKS4 in transgenic red raspberry.
Bar 1 is the expression of RinPKS4 gene in p3301-121 transgenic strain; bar 2 is the expression of RinPKS4 gene in p3301-121-RinPKS4 transgenic lines.
Fig 10.
Schematic diagram of phenylpropanoid pathway; red and green arrows indicate the up regulation of gene expression and the down regulation of gene expression and the width of the arrows as a more visual representation of the fold change in gene expression.
A: General phenylpropanoid pathway; B: Lignin pathway; C: Anthocyanin pathway; D: flavones pathway.