Fig 1.
Profiles of a hen showing different feather positions and skin redness, and the four regions of interest where redness was sampled.
(a) Illustration of the four region of interest (arrows). (b) Locations where skin redness was sampled (black squares, 10 x 10 pixels squares). (b,c) Location where feathers’ position was observed (blue rectangle). Figures illustrate Eden (Meusienne hen) with (a, b) feathers labelled as sleeked or (c) fluffed and with (a) low or (b, c) high skin redness. Pictures are extracted from (a) Resting, (b) Capture, and (c) Dustbathing situations.
Table 1.
Percentage of images where feathers were fluffed depending on the situation.
(a) M-hens. (b) P-hens. A value of 0 means that feathers were sleeked on all the images. Percentages with different letters in a row are significantly different.
Fig 2.
Dendrogram (left hand side of the figures) and table from hierarchical cluster analyses of the redness of the skin for the four ROIs.
(a) M-hens. (b) P-hens. The heatmap contains the tested situations (Alert, Capture, Reward… in red, blue, green…) in rows and ROI (wattles, comb, cheeks, ear lobes) in columns with red colour indicating high-level of redness, blue colour low-level, and yellow colour being between the two. Tables indicate for each situation the number of hens in each cluster (M-hens, n = 7, P-hens, n = 10).
Fig 3.
Median and interquartile range of the redness of the skin for wattles, comb, cheeks and ear lobes depending on the situation.
(a) M-hens. (b) P-hens. Situations with different subscripts differed significantly (GLMM on log2 transformed data with Tukey adjustment for two-by-two comparisons).
Fig 4.
Redness (median and interquartile range) of the skin for wattles, comb, cheeks and ear lobes depending on the arousal of the situation.
(a) M-hens. (b) P-hens. Low arousal includes Feeding, Resting and Preening; High arousal includes Capture, Alert, Dustbathing and Reward. ****: p<0.001 (GLMM on log2 transformed data with Tukey adjustment for two-by-two comparisons).
Fig 5.
Redness (median and interquartile range) of the skin for wattles, comb, cheek and ear lobes depending on the valence and arousal of the situation.
(a) M-hens. (b) P-hens. Negative valence / high arousal (V-/A+) includes Capture and Alert, positive valence / high arousal (V+/A+) includes Dustbathing and Reward, positive valence / low arousal (V+/A) include Feeding, Resting and Preening). Situations with different subscripts differed significantly (GLMM on log2 transformed data with Tukey adjustment for two-by-two comparisons).