Fig 1.
Scatter and violin-strip plots of the DNA concentrations at T1.
Scatter plot of Qubit−DeNovix (Panel A), Qubit−NanoDrop (Panel B) and DeNovix−NanoDrop (Panel C). The multiple R-squared (R2), Spearman correlation (RS), and corresponding p-values are provided. The line of equality is presented as a diagonal line and the dashed line corresponds to the linear regression model with formula as indicated. Violin-strip plots of the DNA concentrations at T1 by DeNovix, NanoDrop, and Qubit (Panel D). Statistically significant differences (Wilcoxon test, p<0.001) are marked with **. Dav: DeNovix mean DNA concentrations, NDav: NanoDrop mean DNA concentrations, Qav: Qubit mean DNA concentrations,T1: prior to freeze storing.
Fig 2.
Scatter and violin-strip plots of the DNA concentrations at T2.
Scatter plot of Qubit−DeNovix (Panel A), Qubit−NanoDrop (Panel B) and DeNovix−NanoDrop (Panel C). The multiple R-squared (R2), Spearman correlation (RS), and corresponding p-values are provided. The line of equality is presented as a diagonal line and the dashed line corresponds to the linear regression model with formula as indicated. Violin-strip plots of the DNA concentrations at T2 by DeNovix, NanoDrop, and Qubit (Panel D). Statistically significant differences (Wilcoxon test, p<0.001) are marked with **. Dav: DeNovix mean DNA concentrations, NDav: NanoDrop mean DNA concentrations, Qav: Qubit mean DNA concentrations, T2: after freeze storing.
Fig 3.
Scatter plot of the ratio of mean DNA concentrations measured by DeNovix and Qubit in function of the DeNovix A260/280 purity ratio measured at T1.
The plot area between 1.7 and 2.0 on the x-axis is highlighted and depicts the interval where DNA is regarded as pure. A more detailed view of this area is shown as an inset plot.
Fig 4.
Violin-strip plots of the A260/280 and A260/230 ratio.
The A260/280 and A260/230 ratio for each timepoint and quantification platform is depicted in Panel A and Panel B, respectively. For each ratio, the proposed reference interval is indicated where contamination is minimal (A260/280: [1.7,2.0], A260/230: [2.0,2.2]). Statistically significant differences (Wilcoxon test, p<0.001) are marked with **. D280av: DeNovix mean values A260/280, ND280av: NanoDrop mean values A260/280. D230av: DeNovix mean values A260/230, ND230av: NanoDrop mean values A260/230. T1: prior to freeze storing, T2: after freeze storing.
Fig 5.
Scatter plots of the A260/280 and A260/230 ratio for T1 (Panel A and B) and T2 (Panel C and D) between DeNovix and NanoDrop. The multiple R-squared (R2), Spearman correlation (RS), and corresponding p-values are provided. The line of equality is presented as a diagonal line. The dashed line corresponds to the linear regression model with formula as indicated. For each ratio, the proposed reference interval is indicated where DNA is regarded as pure (A260/280: [1.7,2.0], A260/230: [2.0,2.2]). T1, prior to freeze storing; T2, after freeze storing.