Fig 1.
Image of a Plasmodium falciparum-infected thin blood smear at 1000x magnification, both without and with the Miller reticle.
At 1000x magnification, one graduation = 1 micrometer. The average size of RBCs is 7μm (ranging from 5 to 10).
Fig 2.
(a) Comparison of the relative standard deviation (RSD) among microscopists, ordered by increasing parasitemia, between two manual techniques, including 10 measurements randomly selected from 10 microscopists, resulted in a mean RSD of 26.40% for the Miller reticle method and 38.22% for the standard measurement method. (b) Distribution of RSD among fields, including the MALARIS estimation on 10 field images. Above each histogram, the measured parasitemia value for each patient, arranged in ascending order of parasitemia, is displayed.
Fig 3.
Repeatability of the parasitemia automatic count by the MALARIS system was assessed on 14 patients with estimated parasitemia from 0.7% to 12.72% and a median value of 2.01%.
The relative standard error rate (RSE) (%) was represented to evaluate the repeatability of k combinations (1 to 5) using 10 thin blood smear images (a). Additionally, a comparison was made with the Miller reticle and Standard manual count (b). The yellow triangles represent the mean value of each pair of measurements.
Fig 4.
Comparison of regression plots for parasitemia estimation.
MALARIS (x-axis) vs. Miller reticle (y-axis) (a), MALARIS (x-axis) vs. flow cytometry (y-axis) (b), and Miller reticle (x-axis) vs. flow cytometry (y-axis) (c). Below the plots, there is a specific focus on the values of parasitemia between 0 and 5%.