Fig 1.
Experimental workflow for ONT sequencing of gDNA extracted from Mtb CPLC samples.
A schematic representation of the experimental workflow, detailing the three sequencing runs, the number of samples multiplexed in each run, and the incorporation of a bead clean-up step for selected samples. The iterative approach involved data analysis after each run to guide adjustments and enhancements in subsequent runs, enabling the optimisation of the ONT sequencing process for Mtb CPLCs. Figure created with Biorender.com.
Table 1.
Performance metrics of multiplexed ONT sequencing runs for Mtb WGS.
Table 2.
Per-sample ONT WGS metrics for Mtb analysis.
Table 3.
Comparison of ONT- and Illumina-based lineage prediction and drug resistance profiling.