Fig 1.
ADAMTSL2 is aberrantly expressed in pan cancer and CRC.
(A) Difference expression of ADAMTSL2 in pan cancer and normal tissues. (B) Difference expression of ADAMTSL2 in CRC and normal colorectal tissues. (C) Difference expression of ADAMTSL2 in CRC and paired normal colorectal tissues. (D) Effectiveness of ADAMTSL2 expression in distinguishing CRC tissues from non-tumor tissues (ROC curve). ns, p ≥ 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
Fig 2.
Correlation of ADAMTSL2 expression with clinical characteristics in CRC.
(A) N stage. (B) Pathological stage. (C) Age. (D) Histological type. (E) Neoplasm type. Significance markers: **, p < 0.01; ***, p < 0.001.
Fig 3.
ADAMTSL2 expression in CRC is associated with a poor prognosis in patients with CRC.
(A) OS. (B) PFS. (C) DSS.
Fig 4.
ADAMTSL2 was an independent variable for predicting OS in CRC.
(A) Forest plot display of the results of the multifactorial Cox regression analysis of ADAMTSL2 and clinical characteristics in CRC. (B) Nomograms were constructed to predict the probability of OS at 1-, 3-, and 5- years in CRC.
Fig 5.
Enrichment analysis of ADAMTSL2 in CRC (GSEA).
Fig 6.
ADAMTSL2 expression was associated with immune infiltration and immune score in CRC.
(A) Lollipop plot. (B) Grouped comparison plots. ns, p ≥ 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
Fig 7.
The expression of ADAMTSL2 in CRC was associated with immune checkpoint genes.
Fig 8.
ADAMTSL2 expression in CRC was associated with TMB/MSI.
(A) TMB. (B) MSI. Horizontal coordinates represent the gene and TMB/MSI correlation coefficients. Vertical coordinates on the graph correspond to various tumors, while the dot sizes represent the magnitude of the correlation coefficients. Furthermore, the colors employed in the schematic indicate the significance of the p-values, with bluer shades indicating smaller p-values.
Fig 9.
ADAMTSL2 expression in CRC was associated with mRNAsi.
The figure depicts the distribution of gene expression on the horizontal axis and the distribution of mRNAsi on the vertical axis; the density curve on the right side represents the trend of the mRNAsi distribution; the density curve on the upper side is the trend of the gene expression distribution; the uppermost values represent the correlation p-value, the correlation coefficient, and the correlation calculation method.
Fig 10.
ADAMTSL2 expression was related to immune infiltration in single CRC cells.
Table 1.
Drug sensitivity analysis of ADAMTSL2.
Fig 11.
Somatic mutations of ADAMTSL2 in CRC.
(A) Lollipop plot showing the distribution of mutations of the ADAMTSL2 gene. (B) Oncoplot showing the somatic landscape of ADAMTSL2 in the CRC cohort. Genes are sorted by mutation frequency and samples are sorted by disease histology, as indicated in the annotation column (bottom). The sidebar plot shows the transformed log10 Q values estimated by MutSigCV. Mutation information for each gene in each sample is shown in the waterfall plot, where the different colors with specific annotations at the bottom indicate the various types of mutations. The vignettes above the legend show the number of mutation burdens. (C) Cluster summary plots show the distribution of variants according to variant classification, type, and SNV category. The bottom (from left to right) indicates the mutational load for each sample (variant classification type). The stacked bar graph shows the top ten mutated genes.
Fig 12.
ADAMTSL2 was aberrantly expressed in the CRC of GSE71187.
Fig 13.
ADAMTSL2 is aberrantly expressed in CRC cell lines.