Table 1.
Distribution of participants based on ApoE4 and cognitive status under female and male cohorts.
Fig 1.
Grey matter AT8 positive cells over the progression of Tau phosphorylation and Aβ aggregation, and their associations with ApoE4 and cognitive status.
(A) Total number of AT8’s progression in correlation with Braak stages. (B) The association between Ripa immunoassay of p-Tau level and the total number of AT8 under different sex groups. (C) The association between Guhcl protein precipitation of p-Tau level and the total number of AT8 in different sex groups. (D) Total number of AT8’s progression in correlation with Thal stages. (E) The association between Ripa immunoassay of Aβ42 level and the total number of AT8 in different sex groups. (F) The association between Guhcl protein precipitation of Aβ42 level and the total number of AT8 in different sex groups. (G) ApoE4 and cognitive status influence on AT8 cells aggregation under different sex groups. (H) ApoE4and cognitive status influences on p-Tau levels (Ripa and Guhcl) under different sex groups. Pearson’s R correlation was conducted B-C, and E-F. Two-way ANOVA and Tukey’s HSD post-hoc analysis was used for G and H. Data represent mean ± SEM. * p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Fig 2.
Grey matter 6e10 positive cells over the progression of Tau phosphorylation and Aβ aggregation, and their associations with ApoE4 and cognitive status.
(A) Total number of 6e10’s progression in correlation with Braak stages. (B) The association between Ripa immunoassay of p-Tau level and the total number of 6e10 under different sex groups. (C) The association between Guhcl protein precipitation of p-Tau level and the total number of 6e10 different sex groups. (D) Total number of 6e10’s progression in correlation with Thal stages. (E) The association between Ripa immunoassay of Aβ42 level and the total number of 6e10 in different sex groups. (F) The association between Guhcl protein precipitation of Aβ 42 level and the total number of 6e10 in different sex groups. (G) ApoE4 and Cognitive status influences on 6e10 cells aggregation under different sex groups. (H) ApoE4 and Cognitive status influences on Aβ42 levels (Ripa and Guhcl) under different sex groups. Pearson’s R correlation was conducted B-C, and E-F. Two-way ANOVA and Tukey’s HSD post-hoc analysis was used for G and H. Data represent mean ± SEM. * p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Fig 3.
Grey matter Iba1 activation over the progression of Tau phosphorylation and Aβ aggregation, and their associations with ApoE4 and cognitive status.
(A) Total number of Iba1’s progression in correlation with Braak stages. (B) The association between Ripa immunoassay of p-tau level and the total number of Iba1 under different sex groups. (C) The association between Guhcl protein precipitation of p-Tau level and the total number of Iba1 different sex groups. (D) Total number of Iba1’s progression in correlation with Thal stages. (E) The association between Ripa immunoassay of Aβ42 level and the total number of Iba1 in different sex groups. (F) The association between Guhcl protein precipitation of Aβ42 level and the total number of Iba1 in different sex groups. (G) ApoE4 and Cognitive status influences on Iba1 cells aggregation under different sex groups. Pearson’s R correlation was conducted B-C, and E-F. Two-way ANOVA and Tukey’s HSD post-hoc analysis was used for G. Data represent mean ± SEM. * p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Fig 4.
Grey matter GFAP activation over progression of Tau phosphorylation and A
β aggregation, and their associations with ApoE4 and cognitive status. (A) Total number of GFAP’s progression in correlation with Braak stages. (B) The association between Ripa immunoassay of p-tau level μm and the total number of GFAP under different sex groups. (C) The association between Guhcl protein precipitation of p-Tau level and the total number of GFAP different sex groups. (D) Total number of GFAP’s progression in correlation with Thal stages. (E) The association between Ripa immunoassay of Aβ42 level μm and the total number of GFAP in different sex groups. (F) The association between Guhcl protein precipitation of Aβ42 level and the total number of GFAP in different sex groups. (G) ApoE4 and Cognitive status influences on GFAP cell aggregation under different sex groups. Pearson’s R correlation was conducted B-C, and E-F. Two-way ANOVA and Tukey’s HSD post-hoc analysis was used for G. Data represent mean ± SEM. * p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Fig 5.
Grey matter NeuN number over the progression of Tau phosphorylation and Aβ aggregation, and their associations with ApoE4 and cognitive status.
(A) Total number of NeuN’s progression in correlation with Braak stages. (B) The association between Ripa immunoassay of p-tau level and the total number of NeuN under different sex groups. (C) The association between Guhcl protein precipitation of p-Tau level and the total number of NeuN different sex groups. (D) The total number of NeuN’s progression is in correlation with the Thal stages. (E) The association between Ripa immunoassay of Aβ42 level and the total number of NeuN in different sex groups. (F) The association between Guhcl protein precipitation of Aβ42 level and the total number of NeuN in different sex groups. (G) ApoE4 and Cognitive status influences on NeuN cell aggregation under different sex groups. Pearson’s R correlation was conducted B-C, and E-F. Two-way ANOVA and Tukey’s HSD post-hoc analysis was used for G. Data represent mean ± SEM. * p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Fig 6.
Receiver Operating Characteristics (ROC) curves for different diagnostic ratios in predicting AD dementia.
The curves represent the performance of the pTau/Aβ42 ratio (blue), tTau/Aβ42 ratio (green), pTau/tTau ratio (red), and Aβ42/40 ratio (orange). The area under the curve (AUC) for each ratio is indicated in the legend. The grey dashed line represents the no-discrimination line, where the classifier has no predictive ability. (A) ROC curves for the pTau/Aβ42, tTau/Aβ42, pTau/tTau, and Aβ42/40 ratios, measured using the Ripa immunoassay, to predict AD dementia. (B) ROC curves for the pTau/Aβ42, tTau/Aβ42, pTau/tTau, and Aβ42/40 ratios, measured using the Guhcl protein precipitation, to predict AD dementia. (C) Spearman correlation between ranked MMSE scores and pTau/Aβ42, tTau/Aβ42, pTau/tTau, and Aβ42/40 ratios under Ripa immunoassay. (D) Spearman correlation between ranked CASI scores and pTau/Aβ42, tTau/Aβ42, pTau/tTau, and Aβ42/40 ratios under Ripa immunoassay. (E) Spearman correlation between ranked MMSE scores and pTau/Aβ42, tTau/Aβ42, pTau/tTau, and Aβ42/40 ratios under Ripa immunoassay. (F) Spearman correlation between ranked CASI scores and pTau/Aβ42, tTau/Aβ42, pTau/tTau, and Aβ42/40 ratios under Guhcl protein precipitation.
Fig 7.
Spearman’s correlation of individual biomarkers and cognitive MMSE and CASI scores under the influence of ApoE4 status.
A) Spearman correlation between ranked MMSE scores and pTau, Aβ42, tTau, and Aβ40 under Ripa immunoassay. (B) Spearman correlation between ranked CASI scores and pTau, Aβ42, tTau, and Aβ40 under Ripa immunoassay. (C) Spearman correlation between ranked MMSE scores and pTau, Aβ42, tTau, and Aβ40 under Guhcl protein precipitation. (D) Spearman correlation between ranked CASI scores and pTau, Aβ42, tTau, and Aβ40 under Guhcl protein precipitation.
Fig 8.
Clustered heatmaps of all pathological biomarkers intercorrelations.
Pearson correlation was conducted and labeled with each of the biomarkers represented on the Heatmap. Correlation scale from -1 to 1, that -1 means the least correlated and 1 means the most correlated, indicating by color from blue to red. (A) heatmap of biomarker and antibodies in grey matter with correlation in white font and p value in green labeled in each box. (B) Clustered heatmap of biomarker and antibodies in grey matter with correlation in black font labeled in each box.