Fig 1.
An overview of the snail-shaped mouse cochlea (center and left panels) and the internal architecture of the cochlea (right panel) are shown. A glass micropipette was inserted through a round window into the scala tympani to collect the perilymph fluid (center and right panels).
Fig 2.
Auditory brainstem response (ABR) thresholds of wild-type (WT) and AppNL-G-F/NL-G-F (KI) mice.
WT and KI mice at 3, 6, and 12 months of age (n = 8 for each group) were subjected to ABR measurements, and auditory thresholds at 4, 8, 16, and 32 kHz were determined. All the data recorded at each frequency are presented in a panel with mean ± SEM. Statistics: one-way ANOVA (F5,42 = 16, p = 1.1 ×10−8 for 4 kHz; F = 16, p = 6.7 ×10−9 for 8 kHz; F = 15, p = 2.2 ×10−8 for 16 kHz; F = 9.9, p = 2.8 ×10−6 for 32 kHz) with Bonferroni post-hoc test (p = 1 for all comparisons between WT and KI with the same age and the frequency).
Fig 3.
Protein content of the perilymph obtained from WT and KI mice.
The concentrations of the perilymph from WT and KI mice at 3, 6, or 12 months of age (n = 4 samples for each group) were measured; all the data are plotted in each panel with mean ± SEM. Statistics: two-tailed Student’s t-test (p = 0.65, 3 months; p = 0.045, 6 months; p = 0.78, 12 months). *p < 0.05.
Fig 4.
Comparison of the landscape of the perilymphatic proteins between WT and KI mice.
(A) Schematic illustration of tandem mass tagging (TMT) proteomic analysis of the perilymph samples. (B) Volcano plots displaying the differentially abundant proteins in the perilymph of KI and WT mice. The horizontal dotted line represents the cut-off p-value (0.05), whereas the vertical dotted lines show cut-off thresholds for downregulated (0.67) and upregulated proteins (1.5). (C and D) List and characterization of the quantitively changed proteins in KI mice compared to that in WT mice. The names of the up- or downregulated proteins are shown in panel C. In panel D, these proteins were classified in terms of the localization to the five different categories according to the annotation in UniProt.
Fig 5.
Protein profiles of the perilymph and cerebrospinal fluid (CSF) in KI mice.
(A) Venn diagrams showing the numbers of up- or downregulated proteins in perilymph or CSF (left: upregulated, right: downregulated). (B and C) List and characterization of the quantitively changed proteins in the CSF of KI mice compared with that in WT mice. The names of up- or downregulated proteins are displayed in panel B. In panel C, the proteins are classified in terms of localization into five different categories according to the annotation in UniProt. The data are derived from the work of Jiang et al. [26].