Fig 1.
Overview of human-derived collagen hydrogel preparation and properties.
(a) Complete process of cHG+abx fabrication. (b) SEM image of the surface of crosslinked hydrogel. (c) Pharmacodynamics of clindamycin elution from cHG over a 72-hour period measured by LCMS. Panel A created with BioRender.com.
Fig 2.
(a) Representative images of the zone of inhibition (ZOI) after indicated hours of pre-elution. (b) Quantification of bacterial ZOI post-treatment with cHG+abx pre-eluted for up to 60 hours, compared to a no-treatment control. One-way ANOVA followed by Tukey’s test was used to establish significance. * indicates p-value < 0.05, ** indicates p-value < 0.01.
Fig 3.
cHG+abx interference of bacterial biofilms.
(a) Visualization of bacterial biofilms after 12 hours of topical treatment, compared to a no-treatment control. (b) Quantification of bacterial density stained with SYTO-61 in developed biofilms over 12 hours of topical treatment. (c) Absorbance (595 nm) of crystal violet solution from biofilms treated with cHG+abx pre-eluted for various timepoints, compared to control. One-way ANOVA followed by Tukey’s test was used to determine significance for both (b) and (c). * indicates p-value <0.05, ** indicates p-value < 0.01.
Fig 4.
LIVE/DEAD quantification of human and mouse cell lineages after up to 72 hours of topical treatment of cHG+abx, compared to a no-treatment control of hFB.
Fig 5.
cHG+abx efficacy in an ex vivo fibroblast (FB) scratch model.
(a) MRSA-colonized FB scratch assay imaged over 48 hours. Non-infected FBs treated with cHG alone (2nd row) show similar growth compared to control (1st row, untreated, non-infected FBs). FBs infected with MRSA without treatment (4th row) showed significant FB death compared to infected FBs treated with cHG + abx (3rd row). (b) Quantification of cell density within scratches of the modified scratch assay over 48 hours of migration. Two-way ANOVA with Tukey’s post-hoc test was used to establish statistical significance between experimental groups. * indicates p-value < 0.05, ** indicates p-value < 0.01, **** indicates p-values < 0.0001.