Fig 1.
Concentration of (a) Cyanidin 3-O-glucoside (C3G) concentration and (b) total γ-oryzanol concentration in fourteen black rice genotypes at 50 to 60 days after flowering. Vertical error bars represent standard error (n = 3). Bars that do not share a common letter are significantly different (p<0.05). A mean value of zero for C3G (i.e., no bar) indicates concentrations were below the C3G detection limit of 0.43 μg/mL.
Fig 2.
Changes in (a) Rice grain moisture content, (b) fresh grain weight, and (c) dry grain weight in genotypes SCU212 and SCU254 during grain development. Vertical error bars represent standard error (n = 6). Means for a given genotype that do not share a common letter are significantly different (p<0.05). Grey dotted line represents genotype SCU212 and solid black line represents genotype SCU254.
Fig 3.
Concentration of (a) Cyanidin 3-O-glucoside (C3G) concentration, b) Cyanidin 3-O-glucoside (C3G) content, (c) Peonidin3-O-glucoside (P3G) concentration, (d) Peonidin 3-O-glucoside (P3G) content, (e) Total γ-oryzanol concentration and (f) Total γ-oryzanol content in SCU212 and SCU254 black rice genotypes in rice grain during grain development stages. Results represent mean value ± SE from six independent biological replicates. Means for a given genotype that do not share a common letter are significantly different (p<0.05). Grey dotted line represents genotype SCU212 and solid black line represents genotype SCU254.
Fig 4.
Relative expression level of genes involved in the MBW complex for anthocyanin regulation in rice genotypes using 2-ΔΔCT a) OsKala3, b) Oskala4, c) OsTTG1 expression levels. Results represent mean values ± SE from three independent biological replicates. Means for a given genotype that do not share a common letter are significantly different (p<0.05). The grey bar represents observations for genotype SCU212, and the black bar represents observations for SCU254.