Fig 1.
Stem cell (A) transplantation (intraperitoneally) (B) of donor derived stem cells into triploid white catfish (Ameiurus catus). Transplanted stem cells migrate to the genital ridge of the recipient, are incorporated, and initiate oogenesis or spermatogenesis. Gonadal growth of the xenogeny (C). The non-injected control treatment (D) showed no fluorescence, while those injected with channel catfish (Ictalurus punctatus) stem cells were fluorescing in fry sampled at 45 (E) and 90 DPH (F).
Table 1.
Primers used for genes [Fst (follistatin) and Hamp (hepcidin antimicrobial protein)] to differentiate channel catfish (Ictalurus punctatus) and blue catfish (I. furcatus).
Fig 2.
Total length (A, C) and weight (B, D) at 45 (●) and 90 (★) days post-hatch (DPH) of triploid xenogenic white catfish (Ameiurus catus) injected with blue catfish (Ictalurus furcatus; A, B) or channel catfish (I. punctatus; C, D) stem cells from 0 to 12 DPH. “CR” represents the non-injected control. Days of injection = Days post hatch.
Fig 3.
Percent survival at 45 (A, C) and 90 days post-hatch DPH (B, D) of triploid xenogenic white catfish (Ameiurus catus) fry injected with blue catfish (Ictalurus furcatus; A, B) or channel catfish (I. punctatus; C, D) stem cells from 0 to 12 days post-hatch (DPH). “CR” represents the non-injected control. Days of injection = Days post hatch.
Fig 4.
Percent cell area and cluster area at 45 (A, B) and 90 days post-hatch (DPH; C, D) of triploid white catfish (Ameiurus catus) fry injected with blue catfish (Ictalurus furcatus) stem cells from 0 to 12 DPH. Days of injection = Days post hatch.
Fig 5.
Percent cell area and cluster area at 45 (A, B) and 90 days post-hatch (DPH; C, D) of triploid white catfish (Ameiurus catus) fry injected with channel catfish (Ictalurus punctatus) stem cells from 0 to 12 DPH. Days of Injection = Days post hatch.
Fig 6.
Sample results from PCR for detecting blue catfish (Ictalurus furcatus) donor cells in the testes of triploid white catfish (I. punctatus).
Blue catfish and white catfish cells were differentiated with PCR using follistatin (Fst) and hepcidin antimicrobial protein (Hamp) genes as markers. W = white catfish control, BL = blue catfish control, CH = channel catfish control, HY = channel -blue hybrid, M = Marker.