Fig 1.
A schematic of the SIM instrumentation used to image samples in this study.
Explanations of abbreviations used in this schematic are: BE- beam expander, LC- light collimator, PBS- polarized beam splitter, SLM- spatial light modulator, P- polarizer, F- filter, and DBS- dichroic beam splitter. This figure was created with BioRender.com.
Table 1.
Summary of patient demographics for samples collected for this study including age, biopsy guidance method, side of body, and suspicion of lymph node involvement.
An expanded table of patient details for each sample is provided in S1 Table.
Fig 2.
An ultrasound guided breast biopsy with invasive ductal carcinoma imaged with a 10X objective on SIM (A), and the subsequent histology slide from the biopsy (B). A stereotactic breast biopsy imaged with a 20X objective on SIM (C), and the subsequent histology slide from the biopsy (D). A zoomed in selection demonstrates the large nuclei of unstructured invasive ductal carcinoma cells found in both SIM images and histology slides (E, F). The ant-like morphology of healthy breast stroma can be seen in selected zooms on SIM images and histology slides from breast biopsies (G, H).
Fig 3.
High resolution images of common breast structures from whole SIM images of breast biopsies and subsequent histology slides.
Scale bars represent 50 μm.
Table 2.
Confusion matrix for the pathologist rater and diagnostic results summary of SIM images against gold-standard histopathology slides.
Positive predictive value is abbreviated PPV and negative predictive value is abbreviated NPV in this table.