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Fig 1.

Photography of Cyphocharax magdalenae, a neotropical freshwater fish.

Courtesy of Jorge E. García-Melo and Luis J. García-Melo, Proyecto CaVFish Colombia.

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Fig 2.

Sampling sites (stars) of Cyphocharax magdalenae in the lower section of the Cauca River (S4-S8).

Self-made creation of the map based on contour lines scaled 1:100,000 from the Instituto Geográfico Agustín Codazzi source, 2019 (Available from: IGAC Geoportal, https://geoportal.igac.gov.co/contenido/datos-abiertos-cartografia-y-geografia).

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Table 1.

Primer sequences and features of 16 microsatellite loci selected for Cyphocharax magdalenae.

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Table 2.

Genetic diversity of Cyphocharax magdalenae in five sections (S4, S5, S6, S7 and S8) of the Cauca River in Colombia.

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Table 3.

Bottleneck tests and effective population size (Ne) of Cyphocharax magdalenae in the five sampling sections and genetic stocks of the Magdalena-Cauca basin.

Statistical significance is noted in bold (P<0.05).

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Table 4.

Estimation of the change in HE of Cyphocharax magdalenae in 10, 50 and 100 generations.

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Fig 3.

Results of Bayesian inference of structure (a) and discriminant analysis of principal components of Cyphocharax magdalenae: (b) Five sections evaluated (c) Two genetic stocks.

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Table 5.

Pairwise comparisons of the genetic structure estimators of Cyphocharax magdalenae.

Jost’s DEST (above diagonal) and F’ST (below diagonal).

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Fig 4.

Temporal structure of Cyphocharax magdalenae during 2019–2021 in the middle and lower sections of the Cauca River (A) and percentage of each stock by season and year of capture (B).

Rainy season (H): H_20: October 2020; H_21(1): April 2021; H_21(2): December 2021; Dry season (S): S_19: July-August-September 2019; S_20: February 2020; S_21: January 2021.

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