Fig 1.
rt-PA-induced lysis of different clot sizes.
Clots of indicated size were aged for 5 hours and treated with 1.3 mg/l rt-PA in PBS for 1 hour. Clot degradation was expressed as a mass loss against control (A) and release of red blood cells–haemoglobin absorbance (B). The box plots (square–mean, horizontal line–median, box–interquartile range, whisker–data range) represent data out of at least three biological replicates each containing three parallels. Significant differences (ANOVA, p < 0.05) are indicated. Further p values: 30 vs 50 μl p = 0.28, 30 vs 90 μl p = 0.07, 50 vs 90 μl p = 0.34 and 90 vs 150 μl 0.09 in (A) and 50 vs 90 μl p = 0.58, 50 vs 150 μl p = 0.82, 90 vs 150 μl 0.49 in (B).
Fig 2.
The dependency of thrombolysis on rt-PA concentration.
Clots with size 50 μl were aged for 5 hours and treated with indicated concentration of rt-PA in PBS for 1 hour. Clot degradation was determined by mass loss (A) and by the release of red blood cells–haemoglobin absorbance (B). The box plots (square–mean, horizontal line–median, box–interquartile range, whisker–data range) represent data out of at least three biological replicates each containing three parallels. Significant differences (ANOVA, p < 0.05) are indicated.
Fig 3.
Degradation of clots of different ages.
Clots with size 50 μl were aged for indicated times and incubated in PBS (A, B) or with 1.3 mg/l rt-PA in PBS (C, D) for 1 hour. Clot degradation was determined by mass loss (A, C) and by the release of red blood cells–haemoglobin absorbance (B, D). The box plots (square–mean, horizontal line–median, box–interquartile range, whisker–data range) represent data out of three biological replicates each containing three parallels. Significant differences (t-test, p < 0.05) are indicated. Further p values: 0.84 for (C) and 0.16 for (D).
Fig 4.