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Fig 1.

Location of the industrial green spaces investigated in this study and an overview of the surroundings.

The industrial green spaces were located between the area of reclaimed land and the Chita Industrial Road. Dotted lines indicate areas where the Large Japanese field mice (Apodemus speciosus) were trapped. Republished from digital national land information (https://nlftp.mlit.go.jp/ksj/) under a CC BY license, with permission from Geospatial Information Division, Ministry of Land, Infrastructure, Transport and Tourism, the government of Japan, original copyright 3 Feb. 2025.

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Fig 2.

Flowchart showing the process used to identify the food plant resources of the Large Japanese field mice (Apodemus speciosus) in this study.

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Table 1.

Food plant taxa identified using DNA metabarcoding of the partial rbcL region.

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Fig 3.

Non-metric multidimensional scaling (NMDS) plot based on Bray–Curtis and Jaccard indices obtained from rbcL analyses.

To visualize both the Bray–Curtis and Jaccard indices, one outlier was removed from each dataset.

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Fig 4.

Coverage-based rarefaction and extrapolation curve.

Shaded areas represent 95% confidence intervals obtained using a bootstrap method with 1,000 replicates.

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Fig 5.

Comparison of the number of plant taxa per fecal sample identified in different seasons.

The box for each season represents the interquartile range (25th to the 75th percentile), with the median number of taxa for each season indicated by a bold line within the box. The ‘whiskers’ extend to the most extreme values within 1.5 times the interquartile range from the median. Statistically significant differences between the four seasons, as determined by the Steel-Dwass test (P<0.05), are indicated by different letters.

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