Fig 1.
Mating systems utilized to produce crossbred lambs.
(A) It depicts F1 crosses including Texel Tamlet × Moghani (TTM), Texel Dalzell × Moghani (TDM), Booroola Merino × Moghani (BMM) and Booroola Romney × Moghani (BRM). (B) It depicts pure-breeding system to produce purebred Moghani (M) lambs. (C) It depicts in-breeding system to produce inbred F2 lambs from half-sibs, Texel strains (TTMTTM-2, TDMTDM-2) and Booroola strains (BMMBMM-2, BRMBRM-2). (D) It depicts outcrossing system to produce F2 lambs from non-relative parents, Texel strains (TTMTDM-2, TDMTTM-2). (E) It depicts paternal backcrossing system to produce first generation of paternal backcross (PBC1) lambs, Texel strains (TDMM-2, TTMM-2) and Booroola strains (BMMM-2, BRMM-2). (F) It depicts maternal backcrossing system to produce first generation of maternal backcross (MBC1) lambs, Booroola strains (MBRM-2, MBMM-2).
Fig 2.
The electrophoretic result of PCR-RFLP band patterns migrated on agarose gel 3.5%.
(A) PCR amplified BMPR1B gene digested by AvaII (5’-G/GWCC) restriction enzyme for Booroola/FecB mutation (OAR6: 29382188A>G, NC_019463.1 of genome assembly Oar_v3.1). The lanes depict Booroola mutation genotypes including homozygous reference (AA), heterozygous (AG) and homozygous variant (GG). SM: GeneRuler 1 kb Plus DNA Ladder #SM1331. (B) PCR amplified ovine myostatin gene (MSTN) digested by HpyCH4IV (5’- A/CGT) restriction enzyme for MSTN mutation (OAR2: 118150665G>A, NC_019463.1 of genome assembly Oar_v3.1). The lanes depict MSTN mutation genotypes including homozygous reference (GG), heterozygous (GA) and homozygous variant (AA). SM: GeneRuler 1 kb Plus DNA Ladder #SM1331.
Fig 3.
Sheep strains generated through different mating systems.
Twelve second-generation crossbred lamb strains, derived from the Moghani pure breed maternal lineage, include TTMTDM-2, TTMTTM-2, TDMTDM-2, TDMTTM-2, BRMBRM-2, BMMBMM-2, TDMM-2, TTMM-2, BRMM-2, BMMM-2, MBMM-2, and MBRM-2.
Fig 4.
Phenotype characteristic of second-generation crossbreds lambs (F2 and BC1).
(A) The F2 offspring resulting from in-breeding with the homozygous variant (GG) genotype for Booroola/FecB mutation (OAR6: 29382188A>G, NC_019463.1 of genome assembly Oar_v3.1). (B) The F2 offspring resulting from in-breeding with the homozygous variant genotype (AA) for MSTN mutation (OAR2: 118150665G>A, NC_019463.1 of genome assembly Oar_v3.1). (C) The F2 offspring resulting from outcrossing with the homozygous variant genotype (AA) for MSTN mutation (OAR2: 118150665G>A, NC_019463.1 of genome assembly Oar_v3.1). (D) The F2 offspring resulting from outcrossing with the homozygous variant (GG) genotype for Booroola/FecB mutation (OAR6: 29382188A>G, NC_019463.1 of genome assembly Oar_v3.1). (E) The BC1 offspring resulting from initial backcross generation.
Table 1.
Percentage rate of coat color patterns across produced strains of progenies between pure Moghani sheep and second generations of crossbred lambs (F2 and BC1).
Table 2.
Preweaning survival rate (%) across mating systems, sex, introgressed gene, and type of birth in second (F2 and BC1) generations of crossbred lambs.
Table 3.
Predicted means for the type of birth effects on lamb growth traits of pure Moghani sheep and second generations of crossbred lambs (F2 and BC1).
Table 4.
Predicted means for the effects of sex on lamb growth traits and fat-tail measurements in second generations of crossbred lambs (F2 and BC1).
Table 5.
Predicted means for the mating systems on lamb growth traits and fat-tail measurements of pure Moghani sheep and second generations of crossbred lambs (F2 and BC1).
Table 6.
Predicted means for the produced strains of progenies on growth traits and fat-tail measurements between pure Moghani sheep and second generations of crossbred lambs (F2 and BC1).
Table 7.
Predicted means for the genotype of introgressed gene on lamb growth traits between pure Moghani sheep and second generations of crossbred lambs (F2 and BC1).