Fig 1.
Bovine milk sample were obtained from CCT positive bTB breakdown herds to obtain relative sensitivity estimates. Anamnestic and non-anamnestic milk samples were obtained from bTB free herds to determine diagnostic and analytical specificity estimates.
Table 1.
Source and characteristics of individual milk reference samples used in the study.
Table 2.
Diagnostic specificity of the Enferplex Bovine TB antibody test using individual milk samples from UK cattle.
Table 3.
Relative or diagnostic sensitivity of the Enferplex Bovine TB antibody test using individual milk samples from UK cattle.
Table 4.
Comparison of proportions obtained between boosted paired serum and milk samples from CCT positive cattle using the Enferplex Bovine TB antibody test.
Table 5.
Likelihood ratio (LR) for positive (LR+) and negative (LR-) individual milk samples.
Fig 2.
Correlation between paired serum and milk in SICCT positive animals for test antigen PPDb using the Enferplex Bovine TB antibody test.
The relative light unit (RLU) obtained with the blank spot was subtracted from the RLU value obtained from antigen spots to obtain a blanked RLU value for each serum and milk sample. Results obtained from serum are shown on the X axis and from the milk samples are shown on the Y axis. Spearman’s rank correlation coefficient r was 0.909, P < 0.0001.
Table 6.
Spearman’s rank correlation coefficient (ρ), 95% confidence intervals, and associated p-values between serum and milk obtained in the Enferplex Bovine TB antibody test using boosted samples.
Fig 3.
Correlation analysis of the number of test antigens recognised by antibody in paired serum and milk from 199 CCT test positive animals.
A—CCT test positive, Hse setting; B—CCT test positive, Hsp setting; C—CCT test positive with VL, Hse setting; D—CCT test positive with VL, Hsp setting. p—Spearman’s Rank Correlation Coefficient. In all 4 comparisons, P values were < 0.0001.