Fig 1.
Morphological features of A. castellanii viruses isolated from marine sediments in Uranouchi Inlet.
(A) Pa1-1, (B) Pa1-2, (C) Pa1-3, (D) Pa1-4, (E) Pa2-1, (F) Pa6-1, (G) Pa11-1, (H) Mo1-1, (I) Me1-1, (J) Me1-2, (K) Ce2-1, (L) Ce7-1.
Fig 2.
Scanning electron microscopy images of selected A. castellanii viruses isolated in this study.
Left, middle, and right columns represent the images of infected cell, enlarged view of the cell surface, and viral particle, respectively. (A–C) Pa1-1, (D–F) Mo1-1, (G–I) Me1-2, (J–L) Ce7-1. Red arrows indicate viral particles adsorbed to the cell.
Fig 3.
Proteomic tree of 12 A. castellanii viral genomes isolated in this study and 533 related eukaryotic dsDNA viruses.
(A) Whole proteomic tree, including 534 related eukaryotic dsDNA viruses, generated by ViPTree server version 3.5. The dendrogram represents the proteome-wide similarity relationships among the 12 A. castellanii viruses isolated in this study (red branches) and reference viral genomes (black branches). Branch lengths are shown on a logarithmic scale from the root of the entire tree. (B) Inner and outer rings that are outside the dendrogram represent viral family classifications and taxonomic groups of known hosts, respectively. (C) Enlarged view of the proteomic tree that includes the viruses isolated in this study.
Fig 4.
Genome map of pandoraviruses isolated in this study.
(A) Pandoravirus macleodensis relatives, (B) P. dulcis relatives. All alignments are represented by colored lines that show the tBLASTx percent identities between two viral genomes.
Fig 5.
Genome map of mollivirus, megaviruses, and cedratviruses isolated in this study.
(A) Mollivirus sibericum and a relative, (B) megavirus relatives, (C) Cedratvirus A11 and relatives. All alignments are represented by colored lines that show the tBLASTx percent identities between two viral genomes.