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Fig 1.

Schematic diagram on P(3HB) coating procedure.

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Table 1.

The experimental setup included three replicate samples for each treatment, with an additional two concrete samples serving as experimental controls.

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Fig 2.

Filtration tank coupled with sand, coral and protein skimmer respectively.

Samples were placed in a floating tray.

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Fig 3.

Distinctive SEM images showcasing various treatment effects on concrete surfaces.

(A) Untreated green concrete surface, (B) melted 1% w/v P(3HB) and (C) melted 6% w/v P(3HB) concrete sample.

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Fig 4.

FTIR spectra analysis of concrete samples.

(a) Extracted P(3HB) powder, (b) G-11, (c) G-13, (d) G-16, (e) G-61, (f) G-63, (g) G-66, and (h) green concrete control, GO.

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Table 2.

EDX analysis to compare the elemental composition of the surface P(3HB) coatings at various treatment.

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Table 2 Expand

Fig 5.

Comparative analysis of biofilm growth after 28 days of different treatments.

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Fig 6.

Microbial abundance of different treatment.

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Fig 7.

Depolymerase activity of P(3HB)-degrading bacteria as evidenced by clear zone formation.

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Table 3.

Number of P(3HB)-degrading bacteria strains isolated from each treatment.

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Fig 8.

Phylogenetic tree based on 16S rRNA gene sequences comparison among isolated marine P(3HB)-degrading bacteria.

The scale bar represents 2% estimated sequence divergence. Bootstrap values (%) were calculated from 1000 trials. Accession numbers are indicated in parentheses.

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Fig 9.

Schematic diagram of degradation of P(3HB)-coated concrete in marine environment.

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