Table 1.
Exercise flow chart.
Fig 1.
Effects of MPO on muscle function and mean lifespan in Drosophila.
(A) Climbing height changed with age in Drosophila. (B) Climbing the height of 1-week-old Drosophila in 3 seconds. (C) Climbing the height of 3-week-old Drosophila in 3 seconds. (D) Climbing the height of 4-week-old Drosophila in 3 seconds. (E) Climbing the height of 5-week-old Drosophila in 3 seconds. (F) Time to fatigue changed with age in Drosophila. (G) Time to fatigue in 1-week-old Drosophila. (H) Time to fatigue in 3-week-old Drosophila. (I) Time to fatigue in 4-week-old Drosophila. (J) Time to fatigue in 5-week-old Drosophila. (K) The average lifespan. For the climbing index and climbing height measurement, the sample size was about 100 Drosophila for each group. For climbing endurance, the sample size was 15 Drosophila for each group. P-values for climbing endurance curves were calculated by the log-rank test. The 1-way analysis of variance (ANOVA) with least significant difference (LSD) tests was used to identify differences among the groups. Data are represented as means ± SEM. *P<0.05; **P <0.01; ***P <0.001; ns means no significant differences.
Fig 2.
Effects of MPO on muscle function in Drosophila.
(A) SOD activity level. (B) MDA level. (C) The expression of the Psn gene in skeletal muscle. (D) The expression of the Sirt1 gene in skeletal muscle. (E) The expression of PGC-1αgene in skeletal muscle. (F) The expression of the arm gene in skeletal muscle. (G) Transmission electron microscopy of Psn-UAS-OE Drosophila muscle. (H) Transmission electron microscopy of Psn-OE Drosophila muscle. For RT-PCR measurement and ELISA measurement, the sample size was about 50/30 Drosophila’ skeletal muscle for each group. The 1-way analysis of variance (ANOVA) with least significant difference (LSD) tests was used to identify differences among the groups. Data are represented as means ± SEM. *P<0.05; **P <0.01; ***P <0.001; ns means no significant differences. Scale: white line represents 5 μm. Transmission electron microscopy images show that MPO increases the number of mitochondria and myogenic fibers. Yellow arrows indicate mitochondria and white arrows indictea myogenic fibers.
Fig 3.
Effects of endurance exercise on muscle function and mean lifespan in Drosophila.
Climbing height changed with age in Drosophila. (B) Climbing the height of 4-week-old Drosophila in 3 seconds. (C) Climbing the height of 5-week-old Drosophila in 3 seconds. (D) Time to fatigue in 4-week-old Drosophila. (E) Time to fatigue in 5-week-old Drosophila. (F) The average lifespan. For the climbing index and climbing height measurement, the sample size was about 100 Drosophila for each group. For climbing endurance, the sample size was 15 Drosophila for each group. P-values for climbing endurance curves were calculated by the log-rank test. The 1-way analysis of variance (ANOVA) with least significant difference (LSD) tests was used to identify differences among the groups. Data are represented as means ± SEM. *P<0.05; **P <0.01; ***P <0.001; ns means no significant differences.
Fig 4.
Effects of endurance exercise on muscle function in Drosophila.
SOD activity level. (B) MDA level. (C) The expression of the Psn gene in skeletal muscle. (D) The expression of the Sirt1 gene in skeletal muscle. (E) The expression of PGC-1αgene in skeletal muscle. (F) The expression of the arm gene in skeletal muscle. (G) Transmission electron microscopy of Psn-UAS-OE-E Drosophila muscle. (H) Transmission electron microscopy of Psn-OE-E Drosophila muscle. For the climbing index and climbing height measurement, the sample size was about 100 Drosophila for each group. For RT-PCR measurement and ELISA measurement, the sample size was about 50/30 Drosophila’ skeletal muscle for each group. The 1-way analysis of variance (ANOVA) with least significant difference (LSD) tests was used to identify differences among the groups. Data are represented as means ± SEM. *P<0.05; **P <0.01; ***P <0.001; ns means no significant differences. Scale: white line represents 5 μm. Transmission electron microscopy images show that endurance exercise increases the number of mitochondria and myogenic fibers. Yellow arrows indicate mitochondria and white arrows indicate myogenic fibers.
Fig 5.
Effects of MPK on muscle function and mean lifespan in Drosophila.
(A) Climbing height changed with age in Drosophila. (B) Climbing the height of 1-week-old Drosophila in 3 seconds. (C) Climbing the height of 3-week-old Drosophila in 3 seconds. (D) Climbing the height of 4-week-old Drosophila in 3 seconds. (E) Climbing the height of 5-week-old Drosophila in 3 seconds. (F) Time to fatigue changed with age in Drosophila. (G) Time to fatigue in 1-week-old Drosophila. (H) Time to fatigue in 3-week-old Drosophila. (I) Time to fatigue in 4-week-old Drosophila. (J) Time to fatigue in 5-week-old Drosophila. (K) The average lifespan. For the climbing height and lifespan measurement, the sample size was about 100 Drosophila for each group. For climbing endurance, the sample size was 15 Drosophila for each group. P-values for climbing endurance curves were calculated by the log-rank test. The 1-way analysis of variance (ANOVA) with least significant difference (LSD) tests was used to identify differences among the groups. Data are represented as means ± SEM. *P<0.05; **P <0.01; ***P <0.001; ns means no significant differences.
Fig 6.
Effects of MPK on muscle function in Drosophila.
(A) The expression of the Psn gene in skeletal muscle. (B) The expression of the Sirt1 gene in skeletal muscle. (C) The expression of PGC-1αgene in skeletal muscle. (D) The expression of the arm gene in skeletal muscle. (E) Transmission electron microscopy of Psn-UAS-RNAi Drosophila muscle. (F) Transmission electron microscopy of Psn-RNAi Drosophila muscle. For RT-PCR measurement, the sample size was about 50 Drosophila’ skeletal muscle for each group. The 1-way analysis of variance (ANOVA) with least significant difference (LSD) tests was used to identify differences among the groups. Data are represented as means ± SEM. *P<0.05; **P <0.01; ***P <0.001; ns means no significant differences. Scale: white line represents 5 μm. Transmission electron microscopy images show that MPO increases the number of mitochondria and myogenic fibers. Yellow arrows indicate mitochondria and white arrows indicate myogenic fibers.
Fig 7.
Effects of endurance exercise on muscle function and mean lifespan in Drosophila.
(A) Climbing height changed with age in Drosophila. (B) Climbing the height of 4-week-old Drosophila in 3 seconds. (C) Climbing the height of 5-week-old Drosophila in 3 seconds. (D) Time to fatigue in 4-week-old Drosophila. (E) Time to fatigue in 5-week-old Drosophila. (F) The average lifespan. For the climbing height and lifespan measurement, the sample size was about 100 Drosophila for each group. For climbing endurance, the sample size was 15 Drosophila for each group. P-values for climbing endurance curves were calculated by the log-rank test. The 1-way analysis of variance (ANOVA) with least significant difference (LSD) tests was used to identify differences among the groups. Data are represented as means ± SEM. *P<0.05; **P <0.01; ***P <0.001; ns means no significant differences.
Fig 8.
Effects of endurance exercise on muscle function in Drosophila.
(A)The expression of the Psn gene in skeletal muscle. (B) The expression of the Sirt1 gene in skeletal muscle. (C) The expression of PGC-1αgene in skeletal muscle. (D) The expression of the arm gene in skeletal muscle. (E) Transmission electron microscopy of Psn-UAS-RNAi+E Drosophila muscle. (F) Transmission electron microscopy of Psn-RNAi+E Drosophila muscle. For the climbing height and lifespan measurement, the sample size was about 100 Drosophila for each group. For RT-PCR measurement, the sample size was about 50 Drosophila’ skeletal muscle for each group. The 1-way analysis of variance (ANOVA) with least significant difference (LSD) tests was used to identify differences among the groups. Data are represented as means ± SEM. *P<0.05; **P <0.01; ***P <0.001; ns means no significant differences. Scale: white line represents 5 μm. Transmission electron microscopy images show that endurance exercise increases the number of mitochondria and myogenic fibers. Yellow arrows indicate mitochondria and white arrows indicate myogenic fibers.