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Fig 1.

Flowchart of analyses and samples used in this study.

a) Genome in a bottle (GIAB) samples. b) 1000 Genomes project samples. c) Known clinical samples available from Coriell.

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Table 1.

Software for genotyping tandem repeats compared.

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Table 2.

Samples used in this study.

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Table 3.

Comparison of processing times and memory usage of STR-calling software.

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Fig 2.

Comparison of STR calls from ExpansionHunter, HipSTR and GangSTR.

A). Comparison of the HG002 genotypes of the STRs called by both GangSTR and HipSTR. The x -axis represents the call made by GangSTR in comparison to the reference sequence (0 = same as reference sequence, -100 represents 100 fewer repeat units than the reference sequence. The y-axis represents the call made by HipSTR. B) Comparison of the genotypes of the STRs called by either GangSTR or HipSTR compared to ExpansionHunter (x-axis). The dotted red lines shows correlation between the calls compared.

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Table 4.

Percentage of loci called in a shared STR catalogue by GangSTR and HipSTR and ExpansionHunter.

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Table 5.

Mendelian inheritance of STR alleles.

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Fig 3.

Repeat unit size distribution of Mendelian inconsistencies.

a) ExpansionHunter, GangSTR and HipSTR calls that are Mendelian inconsistent as a proportion of motif counts in the catalogue, b) all STRs in the catalogue used by the three software tools.

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Table 6.

Comparison of STR genotype calls at core forensic loci for NA12878.

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Table 7.

Detection of STR expansions at known clinical loci.

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