Fig 1.
Representation of Conventional, PEGylated, targeted and multifunctional liposomes (modified from Nel et al. Bioactive Materials 2023 [4]. Figure created with BioRender).
Fig 2.
Thin-film dispersed hydration method.
Synthesis step by step of liposomes via a thin-film dispersed hydration method (Figure created with BioRender).
Fig 3.
Physical properties of liposomes.
Analysis by dynamic light scattering of liposomal particles, subsequent to synthesis (A). Scanning Electron Microscope image of liposomes (B).
Fig 4.
Extrusion study from 7 to 17 passes: values obtained by dynamic light scattering analysis and compared by bar chart (A and B). Scanning Electron Microscope image of the chosen liposomes, 7 passes, with nanoparticle measurements in nm (C).
Fig 5.
Summary of size, polydispersity index and zeta potential through the weeks in table (A) and bar diagram (B).
Fig 6.
Physical properties of ultracentrifuged liposomes.
Dynamic light scattering analysis of ultracentrifuged liposomes using two different molecular weight filters, 10 kDa and 50 kDa. Both supernatant and recollected liposomes where analysed.
Table 1.
Absorbance and EE% values for the three supernatant solutions.
Fig 7.
Cytotoxicity of plain liposomes with three different glioblastoma cell lines (A). Cytotoxicity of encapsulated PI and unencapsulated liposomes with U-251 MG through the weeks (B and C).