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Fig 1.

The change among the different treatment in WWTPs.

(A) Dynamic changes of the content of DMF and (B) COD; (C) Correlation analysis of DMF and COD; (D) The abundance of 16S rRNA; Influent wastewater, IN; Anaerobic process wastewater, AP; Anoxic process wastewater, ANP; Aerobic process wastewater, AEP; Effluent wastewater, EF.

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Fig 1 Expand

Fig 2.

The abundance of ARGs in WWTPs.

(A) PCA analysis between different samples in WWTPs. (B) the relative abundance of ARGs in WWTPs; (C) the absolute abundance of ARGs in WWTPs; (D) Chord diagram analysis between the sample and ARG subtype; (E) the mechanisms of ARGs variation in WWTPs.

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Fig 2 Expand

Fig 3.

The ARGs changed along the WWTPs process.

(A) venn diagrams analysis between the samples. (B) 53 share genes changed between samples; (C) 18 new generated ARGs changed between samples.

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Fig 3 Expand

Fig 4.

Changes of bacterial community.

(A) PCA analysis between samples base on the OTUs; (B) the structure of bacterial between samples; (C) the variation of bacterial at phylum level between samples. (D) the variation of pathogen at species level between samples.

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Fig 5.

The correlation between ARG and their host bacteria.

(A) the analysis base on Spearman’s correlation between ARGs and their host; network analysis revealing the correlations among the fates of ARGs, MGEs, DMF and bacterial in influent (B) and effluent (C).

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Fig 5 Expand