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Table 1.

Comparative overview of some DEA methods for scRNA-seq data.

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Fig 1.

Research framework of this paper.

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Fig 2.

Schematic of RNA sequencing principle.

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Fig 3.

Mean-variance relationship of real scRNA-seq data.

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Table 2.

Comparison of the three data distributions.

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Table 3.

The simulation data generation scheme for different genes.

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Fig 4.

Mean-variance relationship of simulation dataset.

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Fig 5.

Full flow of scRNA-seq data analysis.

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Table 4.

Confusion matrix of different methods.

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Fig 6.

Positive evaluation indicators of each DEA method on simulated datasets under different biological replications (K).

(a) K = 2. (b) K = 3. (c) K = 4. (d) K = 5.

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Fig 7.

Negative evaluation indicators of each DEA method on simulated datasets under different biological replications (K).

(a) K = 2. (b) K = 3. (c) K = 4. (d) K = 5.

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Fig 8.

ROC curves of each DEA method on simulated datasets under different biological replications (K).

(a) K = 2. (b) K = 3. (c) K = 4. (d) K = 5.

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Table 5.

Comprehensive score of each DEA methods under different biological replications (K).

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Fig 9.

Spectral clustering results of PBMCs.

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Fig 10.

DE genes detected by different DEA algorithms.

(a) Venn diagram. (b) Circos plot.

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Table 6.

Evaluation of different DEA methods on two real scRNA-seq datasets.

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