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Table 1.

Method validation parameters for the quantification of antimicrobial residues.

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Table 1 Expand

Table 2.

Physical parameters with standard deviations measured in 2020, 2021 and 2022 in raw and treated water of NW-E DWPF.

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Table 2 Expand

Table 3.

Physical parameters with standard deviations measured in 2020, 2021 and 2022 in raw and treated water of NW-C DWPF.

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Table 3 Expand

Table 4.

Chemical parameters with standard deviations measured in 2021 and 2022 in raw and treated water of NW-E and NW-C DWPFs.

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Table 4 Expand

Fig 1.

Stacked column illustrating phylum-level changes in bacterial community composition of raw and treated water at NW-E and NW-C DWPFs.

Others consist of phyla with relative abundance < 2.00%.

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Fig 1 Expand

Fig 2.

Stacked column illustrating genus-level changes in bacterial community composition in raw and treated water of NW-E and NW-C DWPFs.

Others consist of genera with relative abundance < 1.00%.

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Fig 2 Expand

Fig 3.

Bacterial alpha diversity indices (A-ACE, B-Chao1, C-Shannon and D-Simpson) in raw and treated water of NW-E and NW-C DWPF.

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Fig 4.

Chord diagram illustrating the end-point PCR screening process of ARGs in raw and treated water of NW-E and NW-C DWPFs.

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Table 5.

Concentrations of ARGs in raw and treated water of NW-E and NW-C DWPFs (units in gene copies/16S Rrna ± standard deviation).

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Table 6.

Concentrations with standard deviations of target antimicrobial residues in raw and treated water of NW-E DWPF.

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Table 6 Expand

Table 7.

Concentrations with standard deviations of target antimicrobial residues in raw and treated water of NW-C DWPF.

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Table 7 Expand

Fig 5.

Heatmap of risk quotients (RQs) in raw and treated water of NW-E and NW-C DWPFs.

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Fig 5 Expand

Fig 6.

RDA of correlation between (A) bacterial community composition, physicochemical parameters, ARGs and antibiotic residues in raw and treated water of NW-E and NW-C DWPFs.

FCZ–fluconazole; temp–temperature; AMP–ampicillin; sal–salinity; COD–Chemical oxygen demand, TDS–Total Dissolved Solids; SMX–Sulfamethoxazole; NO2—nitrite, NO3—nitrate; PO4 –phosphate; TMP–trimethoprim.

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