Fig 1.
Interstrain differences in the volage threshold in LT and HT rat strains.
HT—animals with high excitability threshold (n = 32), LT–animals with low excitability threshold (n = 34); the graphs represent the mean and SEM; **** p< .0001 (unpaired t-test).
Fig 2.
Differences in alpha diversity indices in control groups of HT and LT rat strains.
The horizontal axis on panel A shows the animals of the control groups at the corresponding time after the end of stress in the experimental groups. The horizontal axis on panel B shows the intact animals of the consolidated control. HT–high threshold, low-excitable rats; LT–low threshold, high-excitable rats; the graphs represent the mean and SEM; ** p < 0,01, in A–paired t-test, in B—unpaired t-test.
Fig 3.
Alpha diversity indices in control and experimental groups of HT and LT rats after stress.
The horizontal axis shows the animals of the control groups (n = 18 in HT strain and n = 18 in LT strain) and the experimental groups (n = 6 in each group) at the different time points after the end of stress. HT–high threshold, low-excitable rats; LT–low threshold, high-excitable rats; the graphs represent the mean and SEM; one-dimensional variance analysis with repeated measurements (ANOVA), using the Greenhouse-Geisser correction method; case/control—unpaired t-test p > 0,05.
Fig 4.
Differences in the relative abundance of some bacterial genera in the gut microbiome of the HT and LT rat strains.
HT–high threshold, low-excitable intact rats; LT–low threshold, high-excitable intact rats; n = 18 in each group; the graphs represent the mean and SEM **p<0.01 *p<0.05, unpaired t-test.
Fig 5.
Differences in the relative abundance of top bacterial genera in control and stress groups of HT and LT rat strains.
HT–high threshold, low-excitable rats; LT–low threshold, high-excitable rats; control–rats without stress exposure; before stress–experimental rats before stress exposure; 0, 7, 24 –days after the end of stress exposure in experimental groups; n = 6 in each group; the graphs represent the mean and SEM **p<0.01 *p<0.05, case/control—unpaired t-test; experimental rats in different time points—one-dimensional variance analysis with repeated measurements (ANOVA), using the Greenhouse-Geisser correction method and paired t-test.
Fig 6.
Altered composition in the gut microbiota at phylum levels in stressed groups of HT and LT rat strains.
HT–high threshold, low-excitable rats; LT–low threshold, high-excitable rats; 0, 7, 24 –days after the end of stress exposure in experimental groups.