Fig 1.
Normalized melt curves for the specimens targeting the c.1117G>T variant in exon 8.
Normalized melt curves were showing that the specimens with homozygous and heterozygous states were clearly distinguishable from the wild-type specimens, as manifested by the difference in relative fluorescence unit.
Fig 2.
Difference curves generated by specimens targeting the c.1117G>T variant in exon 8.
Discernable changes in three difference curves were showing that the specimens with homozygous and heterozygous states were clearly distinguishable from the wild-type allele, as manifested by the difference in relative fluorescence unit.
Fig 3.
Normalized melt curves generated by specimens targeting the c.1193G>C variant in exon 8.
Discernable changes in normalized melt curves were showing that the specimens with homozygous (orange color) and heterozygous (green color) states were clearly distinguishable from the wild-type (purple color) alleles, as manifested by the difference in relative fluorescence unit.
Fig 4.
Difference curves for specimens targeting the c.1193G>C variant in exon 8.
Discernable changes in difference curves were showing that the specimens with homozygous and heterozygous states were clearly distinguishable from the wild-type states, as manifested by the difference in relative fluorescence unit.
Fig 5.
Normalized melt curves for specimens targeting the c.2173A>C variant in exon 12.
Discernable changes in normalized melt curves were showing that the specimens with homozygous and heterozygous states were clearly distinguishable from the wild-type alleles, as manifested by the difference in relative fluorescence unit.
Fig 6.
Differential curves for specimens targeting the c.2173A>C variant in exon 12.
Discernable changes in difference curves were showing specimens with homozygous and heterozygous states were clearly distinguishable from the wild-type, as manifested by the difference in relative fluorescence unit.