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Fig 1.

Unidirectional Mendelian randomization model.

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Fig 1 Expand

Fig 2.

Bidirectional Mendelian randomization model with a feedback loop.

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Table 1.

Simulation scenario 1: The unidirectional Mendelian randomization model is used.

Parameter estimates are based on 1000 replicates.

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Table 1 Expand

Fig 3.

Median of absolute bias (MAB) of bidirectional causal effect estimations for simulation scenario 2: Simulation using the bidirectional Mendelian randomization model and strong instrumental variables (IVs).

Parameter estimations are based on 1000 simulation replicates. A: MAB of estimations using one strong IV. B: MAB of estimations using 20 strong IVs. The color bar shows the range of MAB. BiRatio = bidirectional ratio method; BiLIML = limited information maximum likelihood method; LIML = limited information maximum likelihood method.

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Fig 3 Expand

Table 2.

Simulation scenario 2 with strong instrumental variables (IVs): The bidirectional Mendelian randomization model is used.

Parameter estimates are based on 1000 replicates.

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Table 2 Expand

Fig 4.

Median of absolute bias (MAB) of bidirectional causal effect estimations for simulation scenario 3: Simulation using the bidirectional Mendelian randomization model and weak instrumental variables (IVs).

Parameter estimations are based on 1000 simulation replicates. A: MAB of estimations using 20 weak IVs. B: MAB of estimations using 100 weak IVs. The color bar for each figure shows the range of the MAB. BiRatio = bidirectional ratio method; BiLIML = limited information maximum likelihood method.

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Fig 4 Expand

Table 3.

Simulation scenario 3 with weak instrumental variables: The simulation model is the bidirectional Mendelian randomization model.

Parameter estimates are based on 1000 replicates.

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Table 3 Expand

Table 4.

The bidirectional causal effects estimation between body mass index and fasting glucose.

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Table 4 Expand