Fig 1.
(a) 1H-NMR spectrum of P(AAm-co-AN)-CPTD in DMSO-d6 (b) Transmittance curves of P(AAm-co-AN)-CPTD vesicles solution at different temperatures. (c) UV-Vis absorption spectra of AuNRs, AuNRs@CeO2, DOX and PA-DOX, (d) TEM image of PA-DOX (inset is the image of pristine AuNRs), (e) Zeta potential of AuNRs, AuNRs@CeO2 and PA-DOX, (f) DLS curves of AuNRs, AuNRs@CeO2 and PA-DOX.
Fig 2.
Photothermal performance and Drug loading and release.
(a,b) Temperature change curves of PA-DOX solution under different powers NIR 808 nm laser irradiation, (c) Temperature change curve of PA-DOX solution after a single on/off NIR laser irradiation, (d) Relationship between the cooling time and the negative natural logarithm of temperature driving force after the NIR laser turned off, (e) Cumulative drug release curves of PA-DOX in PBS (pH 7.4) at 37°C, 50°C, and under the NIR laser irradiation, (f) Drug release curve of PA-DOX over 300 min under on/off cycles of NIR laser irradiation.
Fig 3.
Effect of PA-DOX on H2O2 decomposition and O2 generation.
(a) Decomposing curves of H2O2 in the presence of AuNR, AuNRs@CeO2, and PA-DOX with or without NIR 808 nm laser at 3.0 W/cm2, (b) Generation curves of O2 in 100 μM H2O2 solution over time under different conditions.
Fig 4.
Effect of PA-DOX on cell viability and migration and in vitro cellular uptake capacity.
(a, b) Cell viability of HepG2 cells treated with AuNRs, AuNRs@CeO2, free DOX and PA-DOX (with/without NIR laser irradiation) at various concentrations at pH 7.4, (c) The images of cells (left to right) after treating with PBS (control), AuNRs, AuNRs@CeO2, free DOX and PA-DOX (with (low row)/without (up row) NIR laser irradiation), (d) Numbers of migrating cells calculated from (c), (e) In vitro cellular uptake of free DOX and PA-DOX (with/without NIR laser irradiation). DOX (red), DAPI (blue), scale bar, 50 μm, (f) CLSM images of H2O2 in HepG2 cells treated with AuNRs (control), AuNRs@CeO2, and PA-DOX (with/without NIR laser irradiation) in the presence of H2O2.
Fig 5.
Anti-tumor effect of PA-DOX in vivo.
(a) Tumor volume of mice treated with different samples, (b) Tumor weight of mice treated with different samples, (c) Photos of tumor tissue from mice treated with different samples, a-f were consistent with Fig 5b, (d) Body weight of mice treated with different groups, (e) H&E of tumor tissue sections from mice treated with different groups.