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Table 1.

Effect of pretreatments on compositional characteristics of Sargassum spp. UT = Untreated; WW = Water washing; S+W = Soaking + Warm water washing; CS = Chemical soaking.

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Fig 1.

Changes in the compositional characteristics (% dry weight) of Sargassum spp. by the effect of pretreatments.

UT = Untreated, WW = Water washing, S+W = Soaking + Warm water washing, CS = Chemical soaking.

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Fig 1 Expand

Table 2.

Influence of pretreatments on the content of mineral salts and total removal in the biomass of Sargassum spp. UT = Untreated, WW = Water washing, S+W = Soaking + Warm water washing, CS = Chemical soaking.

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Table 3.

A summary of the results of the biomethane potential test of untreated and pretreated Sargassum spp. after 70 days of incubation and 53 days for cellulose.

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Table 3 Expand

Fig 2.

Cumulative methane production expressed in N mL·g-1 VS of pretreated and untreated Sargassum spp. biomass after 70 days of incubation.

UT = Untreated, WW = Water washing, S+W = Soaking + Warm water washing, CS = Chemical soaking. Error bars represent the standard deviation of the mean (n = 3).

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Fig 2 Expand

Fig 3.

Heat map of Spearman’s correlation analysis between the compositional characteristics of Sargassum spp. and BMP/BI.

Cel = Cellulose; Hcel = Hemicellulose; Lig = Lignin; TPC = Total phenolic content. The mark * indicates the significance test (p <0.05).

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Fig 3 Expand

Table 4.

Alpha-diversity indices of the different microbial communities.

WW = Water washing, CS = Chemical soaking.

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Table 4 Expand

Fig 4.

Relative abundances of bacterial communities at the phylum (a) and genus (b) level of the inoculum, WW (water washing), and CS (chemical soaking). Taxa with abundances <1% of the total sequences in the samples were grouped as other.

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Fig 4 Expand

Fig 5.

Relative abundances of archaeal communities at the phylum (a) and genus (b) level of the inoculum, WW (water washing), and CS (chemical soaking). Taxa with abundances <2% of the total sequences in the samples were grouped as other.

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Fig 5 Expand