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Fig 1.

DNA barcoding analysis framework.

Basic steps of the DNA barcoding analysis for the identification of samples collected at solar energy facilities. After collection, samples are processed to extract DNA, which is then amplified. PCR products are sequenced and compared to those archived on GenBank through a BLAST search, which leads to a genetic match (positive identification). Steps can be repeated using different barcoding primers when necessary. * It is strongly suggested to verify morphological identifications through the genetic approach (see “Best Practices” below) depicted here, and to include phenological data, particularly when ambiguity exists.

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Table 1.

Categorization of samples identified via genetic approach.

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Table 2.

Discrepancies between morphological and genetic identifications.

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Table 2 Expand

Fig 2.

Proportions of avian communities.

Comparison of the overall proportions of avian community categorized as Terrestrial Birds, Songbirds, and Waterbirds. (A) Proportions are significantly different (χ2 = 57.96, p<0.00001, n = 4,328) between genetic identifications (inner circle) and morphological identifications (outer circle). (B) Differences in avian group proportions across technology type (example photo above each chart). A significant difference (χ2 = 811.84, p<0.00001, n = 4,328) in bird group proportions was observed by technology type.

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