Fig 1.
Details of the experiments with cigarette beetle and microbiome sampling.
Cigarette beetle were initially cultured on wheat as natal (N) source (called as natal phase) for 6 generations (G) and sampled for microbiome analysis at N- 6G. Then natal phase colonies were cultured for another 6G on each of exposed (E) diets called as exposed phase colonies and beetles sampled for microbiome at E-6G. The colonies were continued for a further 6G on wheat diet called as reverted (R) phase with microbiome sampling at R-6G. Six biological replicates were maintained for each treatment and three technical replicates for 16s DNA sequencing.
Fig 2.
Bacterial structure of cigarette beetle microbiome at phylum and genus level.
Bar-plots show the abundance and distribution of the A) 20 most abundant phylum and B) 30 most abundant genera across diets.
Fig 3.
Alpha diversity indices of cigarette beetle microbiome across phases.
Statistically significant values * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.005; df = 2.
Fig 4.
Heat map of the top 35 abundant families showing the relative abundance of the bacteria taxa across diet sources.
Fig 5.
The relative abundance of genus Wolbachia associated with cigarette beetle across diets.
Error bars are indicated, lowercase letters indicate homogenous groups (Tukey, p ≤ .05; df = 8).
Fig 6.
Principal coordinate analysis (PCoA) of community structure of cigarette beetle across diet sources (df = 8) and phases (df = 2).
Each symbol represents phase and corresponding color represents diet. Colored triangle represents natal phase; colored inverted triangle, diamond, circle, square represents exposed phase; colorless inverted triangle, diamond, circle and square represents reverted phase.
Fig 7.
Venn diagram representing the shared genus of cigarette beetle microbiome across diet source.
Fig 8.
Comparison on the relative abundance of key genera associated with the microbiomes of cigarette beetle from natal phase and reverted phase after six generations.
Statistically significant values * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.005; df = 4.
Table 1.
Proportion taxa of cigarette beetle (excluding Wolbachia) observed among diet sources at different phases and that has contributed > 0.12% to the entire data set at the genus level.