Fig 1.
Thrombin generation in Vacutainer and Monovette tubes, and Monovette tubes after addition of CTI.
A shows the thrombin generation at low TF concentrations in plasma from Vacutainer tubes (blue shades) and Monovette tubes (red shades). The dashed lines are samples with antibodies against TF (aTF). B shows the thrombin generation at low TF concentrations in samples from Monovette tubes with 18.3 μg/mL corn trypsin inhibitor (CTI) added.
Fig 2.
A calibration curve for TF concentration.
A shows a calibration curve for the TF concentration vs the Lagtime (and the resulting equation), and B shows that the relationship is linear in a log/log system.
Fig 3.
Linearity of dilutions of TF in an LPS stimulated sample. The fig depicts the measured TF concentrations of dilutions of a plasma (having a concentration of 0.8 pM TF) from a blood sample which has been stimulated with LPS. The dilution curve is linear.
Fig 4.
Measurements of TF in samples.
A depicts the thrombin generation measured in EV samples from 4 normal controls having 1 (red, n-EV3), 2 (blue, nEV2), 3 (black, n-EV1) and 6 fM TF (purple, n-EV6). The EVs are concentrated from 7500 μl plasma sampled resuspended in 300 μl SP before mixing with SP in the TG analysis. The dashed lines show the thrombin generation in samples with aTF added, i.e. there is a small TF independent activation in these samples. B shows the thrombin generation in four LPS stimulated blood samples. EV-TF concentrations (EV5-EV8) were between 300 and 800 fM. Below the figures are shown LT and the corresponding TF activity.
Fig 5.
Effect of an increased concentration of phospholipid.
Thrombin generation in samples where the phospholipid (PPL) concentration is increased from the normal level (4 μM) to 5, 6 or 8 μM. The TF concentrations were from 0.01 to 0.1 pM. Below the figures are shown LT. At the higher concentrations of TF the effect of PL is negligible whereas there is a small effect at the lower concentrations.