Fig 1.
Shannon diversity index and the point and gaze test of joint attention.
Microbial diversity analysis measured with Shannon index. Comparisons between infants who were unsuccessful versus successful on the point subtest (A), gaze subtest (B), or at least one of the point and gaze subtests (C), as well as between the collection site (UBC and McMaster) (D). Two-tailed t-tests were performed between the conditions. No statistical differences were observed between the groups.
Fig 2.
(A) PCA analysis on microbiome abundance data. Data was CLR transformed before the analysis. Success at least one of the point and gaze subtest tests are indicated in red and failure on both in green (95% confidence ellipse assuming a multivariate normal distribution) and shape indicates the sample site. (B) PCA analysis on the microbiome abundance data visualizing the top five contributing bacterial species (percentage %). Contribution is calculated as the cos2 of the variable divided the total cos2 of a given principal component: (var.cos2*100)/(total cos2 of the component).
Fig 3.
Bacteria relative abundance at the phylum level. A) Difference in bacteria relative abundance by success on at least one of the point and gaze subtests versus not successful on both B) Violin plot of the relative abundance difference between collection site (McMaster and UBC) and joint attention test results.
Fig 4.
(A) Association analysis between bacteria abundance (CLR transformed) and EEG measurements, at the genus level. (B) Association analysis between unstratified pathways abundances and EEG measurements. Significant associations have an adjusted p-value < 0.05 and a minimum prevalence of 10%.