Fig 1.
M2b macrophage injection attenuates DOX-induced cardiac injury in mice.
(A) Schematic of the protocol for animal experiment; (B) M2b macrophage improves mortality rate in DOX-induced cardiac injury mice. Kaplan-Meier survival curves in the group (n = 5 for each group); (C) Echocardiography of mice; (D-E) Left ventricular ejection fraction (LVEF) and fractional shortening (LVFS) in the DOX group(n = 5) were significantly lower than those in the control group(n = 5). Both LVEF and LVFS were higher in the M2b group(n = 5) than those in the DOX group; (F-G) The serum CKMB and LDH levels in the DOX group(n = 5) were significantly higher than those in the control group(n = 5). Both CKMB level and LDH level were relatively lower in the M2b group (n = 5) than those in the DOX group. “*” denoting a p-value less than 0.05.
Fig 2.
M2b macrophage injection attenuates doxorubicin induced acute myocardiocyte injury in vivo.
(A) Representative H&E staining micrograph of heart tissue sections(40×). The scale bar is 100 μm in each panel; (B) Representative images of the TUNEL assay of heart tissue sections (40×). The scale bar is 100 μm in each panel; (C) Representative images of the Transmission electron microscopy of heart tissue sections. The scale bar is 20 μm in each panel.
Fig 3.
M2b macrophage promotes autophagy in DOX-induced cardiomyopathy in vivo.
(A) Western blot of mTOR, p-mTOR, P62, LC3 in hearts, full-length blots are presented in S2 Fig; (B) Qualification of p-mTOR/mTOR expression in the heart (n = 5 for each group); (C) Qualification of P62 expression in the heart (n = 5 for each group); (D) Qualification of LC3 expression in the hearts (n = 5 for each group). “*” denoting a p-value less than 0.05.
Fig 4.
M2b macrophage rescue DOX-induced cardiomyocyte death in an autophagy dependent manner.
(A) Western blot of mTOR, p-mTOR, P62, LC3 in HL-1 cells, full-length blots are presented in S3 Fig; (B) Qualification of p-mTOR/mTOR expression in HL-1 cells (n = 5 for each group); (C) Qualification of P62 expression in HL-1 cells (n = 5 for each group); (D) Qualification of LC3 expression HL-1 cells (n = 5 for each group); (E) CCK-8 assay of HL-1 cells.(5 wells each time for the CCK-8 assay). “*” denoting a p-value less than 0.05. “M2b(H)” indicates the high concentration of M2b supernatant.