Fig 1.
Analysis of differential expression of QKI and pathway enrichment.
Expression of QKI in normal samples and tumour tissue samples from patients with oesophageal carcinoma (normal group, n = 286; tumour group, n = 182) (A). * p.adj < 0.05. GSEA showed the enriched pathway differences between the high and low expression groups of QKI; ESCA: Oesophageal carcinoma (B).
Fig 2.
Screening of circRNAs downstream of the variable shear factor QKI.
Seven kinds of variable clipping UpSet diagrams (A). The network relationship between QKI and its regulatory gene variable splicing pattern (B). Heatmap for differentially expressed genes in the GSE189830 dataset (normal group, n = 3; tumour group, n = 3). (C). The expression values are represented in different colors; red indicates high expression, and green indicates low expression. Darker node colors indicate more extreme high or low expression of the respective gene. Venn diagram of the intersection of 50 coding genes related to QKI and 72 genes encoding differentially expressed circRNAs (D).
Table 1.
Correlation analysis results of variable splicing of QKI and its downstream coding genes.
Fig 3.
Correlation analysis of QKI and EMT-related genes.
Volcano map of TCGA-ESCA dataset differential expression analysis. Red dots represent significantly upregulated genes, blue dots represent significantly downregulated genes, and black dots represent genes with no difference in expression (A). Venn diagram of the intersection of 204 significantly upregulated genes in oesophageal cancer and 120 EMT-activated genes (B). The expression correlation analysis results of QKI and IL-11, MFAP2, MMP10 and MMP1 are presented (C-F).
Fig 4.
Correlation analysis between EMT-related genes and survival of oesophageal cancer patients and QKI expression.
The hsa_circ_0006646 and hsa_circ_0061395 downstream miRNA-mRNA regulatory networks (A-B). The mechanism by which QKI promotes the EMT process by regulating the circRNA-miRNA-mRNA regulatory network (C).
Table 2.
The targets of has_circ_0006646.
Table 3.
The targets of has_circ_0061395.
Table 4.
Binding of miRNA and mRNA.