Fig 1.
The scheme of the chamber for hypothermal storage of frog oocytes in the atmosphere of biologically active gases.
Fig 2.
Percentage of fertilized eggs from the overall oocyte number (A) and percentage of hatched larvae from the overall number of eggs (B) after storage at +4°C.
Each graph point represents a mean value ± SD for frogs (min. 30×3 oocytes). Day 0 –fresh oocytes. The asterisks represent the reliability of differences between the experimental groups and the control group at the same time point of hypothermal preservation: * P = 0.05, ** P = 0.01.
Fig 3.
Stages of development of R. temporaria zygote after storage at 4°С in the atmosphere of biologically active gases CO+O2 for 4 days.
Stages of embryonic development: A. Stage 10 (21 h); B. Stage 22 (47.5 h); С. Stage 24 (53.5 h). Larval development: D. Stage 31 (4.5 days).