Fig 1.
Citrus tristeza virus symptoms on sweet orange trees on citrumelo rootstocks.
Yellowing of leaves (A) andstem pitting and grooving at graft union (B).
Fig 2.
Electrophoresis pattern of RT-PCR products using total RNA extracts.
Lane 1: A healthy orange seedling, Lane 2: Infected trees, Lane 3: CTV positive control, Lane 4: DNA ladder (Thermo Scientific, UK).
Fig 3.
Schematic diagram of the CTV genome organization.
The open boxes represent ORFs and their translation products.
Table 1.
Genome features and ORFs ofCTV sequence of Sari isolate.
Fig 4.
Phylogenetic tree based on full-length genomes of the Sari isolate (Sari, shown with the red circle) of CTV, and the isolates from GenBank shown as accession number and country of origin.
The tree was constructed by the maximum likelihood method using MEGA 11 software. Numbers on branches are bootstrap values of 1,000 replicates. Beet yellows virus was used as an outgroup : T30,
: L1,
: T3,
: HA-65,
: VT,
: T68,
: S1,
: HA-65,
: A18,
: This study,
: RB,
: M1,
: T36,
: Outgroup.
Fig 5.
Expression levels (transcript per million, TPM) of Citrus tristeza virus (CTV) Gene IDs in RNA-seq data of citrus infected with CTV.
Fig 6.
The tertiary structure of polyprotein and location of SNVs (red color).
A and B are figures of polyproteins from different views.
Table 2.
SNVs in the full-length genome of CTV, Sari isolate.