Fig 1.
Schematic of the on-site detection system for Candidatus Liberibacter asiaticus (Las) detection.
Fig 2.
Optimization of concentration of TaqMan probe for iiPCR assay for Candidatus Liberibacter asiaticus (Las).
Different probe concentrations (0 nM to 250 nM) were evaluated the effects of TaqMan probe concentration on production of fluorescent signals. Mean S/N ratio of each reaction was plotted against probe concentration. Each reaction was performed in three replicate reactions. Error bars represent the SDs. S/N ratio, fluorescent signalafter/fluorescent signalbefore; SD, standard deviation.
Table 1.
Analytical specificity analysis of TaqMan probe-iiPCR for Candidatus Liberibacter asiaticus (Las) detection.
Table 2.
Analytical sensitivities of TaqMan probe-iiPCR and real-time PCR for detection of Candidatus Liberibacter asiaticus (Las).
Fig 3.
Dilution ratio evaluation of simple DNA extraction by PCR assay.
The plant 18S rRNA-encoding gene amplicons (67-bp, arrow) were detected in electrophoresis analysis. M, 100-bp DNA ladders (GeneDireX); No, no dilution; 5X, 5-folds dilution; 10X, 10-folds dilution; 20X, 20-folds dilution; 50X, 50-folds dilution; +, DNA isolated by conventional extraction; P, 103 copies of standard template (plasmid containing targeting gene region); NTC, no template control (ddH2O).
Table 3.
Detection of Candidatus Liberibacter asiaticus (Las) in field citrus samples by on-site detection system, TaqMan probe-iiPCR assay and real-time PCR method.
Table 4.
Comparison of diagnostic performances of on-site detection system, TaqMan probe-iiPCR assay with real-time PCR method for Candidatus Liberibacter asiaticus (Las) detection.