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Fig 1.

Appearance of the Rattractor system.

The rat was stimulated in the MFB as it entered the virtual cage indicated with a circle.

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Fig 2.

(a) Stimulator circuit to generate bipolar current stimuli. (b) Electrode unit with two electrode needles and two references.

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Fig 3.

A set of 10 square pulses were generated with our circuit to apply bipolar current stimuli (left); temporal closeup around the first pulse (right).

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Fig 4.

A graphical user interface (GUI) was developed to show the rat’s position (white circle) and heading direction (bar) as well as the stimulated region (i.e., virtual cage), drawn as a dark yellow circle and manually positioned by the experimenter using the mouse cursor.

The red color around the rat position indicates that the subject is electrically stimulated at that time.

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Fig 5.

In each image, a red circle indicates the stimulated site and the gray area surrounded by a blue outline highlights the MFB region superimposed on a coronal section of a stereotaxic atlas image [21].

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Fig 6.

Typical rat trajectory without electrical stimulation in the brain (null condition; left), and a trajectory under stimulation in the MFB as the rat stayed in the virtual cage indicated by a circle (right).

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Fig 7.

Line plots showing the temporal changes of the distance metric d throughout the sessions.

The bar plots underneath compare the count of bins where the distance metric d stayed <0.1 [m]. The analyses were conducted on POW 1 and 2 sessions for the subjects C, F, and I.

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Fig 8.

Line plots showing temporal changes in the distance metric d throughout sessions.

Bar plots at the bottom comparing bin counts where the distance metric d was <0.1 [m]. The analyses were conducted on subjects A, B, E, and H during the POW 1 session.

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Fig 9.

Histogram indicating the time s that the rat stayed on a specific location without stimulation (left), and in the virtual cage indicated by a red circle with stimuli in the MFB (right), respectively.

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Fig 10.

The plot above shows a two-dimensional trajectory of subject C (light blue) and the virtual cage (dark red), which was manually moved.

The numbers show elapsed time s since the beginning of the session. The two plots below show temporal changes in x and y coordinates.

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